DETERMINATION OF OPTIMAL EXTRACTION CONDITIONS OF PHENOLIC COMPOUNDS FROM ACORUS CALAMUS LEAVES

Keywords: Acorus calamus, leaves, extraction, standardization

Abstract

From the beginning of its existence and until now, interest in herbal remedies has only grown. Not surprisingly, medicinal herbs are a source of unique combinations of biologically active components that are capable of exhibiting significant pharmacological effects. One of these medicinal plants is Acorus calamus (sweet flag), whose rhizomes have long been used in official and folk medicine, cooking and cosmetology. However, recent studies point to the considerable potential of the aerial part of Acorus calamus as a novel herbal remedy, which is associated with the presence in the leaf of the plant, in addition to essential oil, a considerable amount of substances of phenolic nature.

The aim of the research was to determine the optimal conditions for the removal of phenolic compounds from the leaves of Acorus calamus and to determine the technological parameters of raw materials.

Materials and methods. During the research, samples of raw materials collected on the territory of Kharkiv region were used, as well as methods that are given in the State Pharmacopoeia of Ukraine.

Result. As a result of the research, the extraction conditions at which there was highest content of the extraction of biologically active substances were determined. The extractant, the degree of grinding of the raw material and the optimum extraction time were selected, which allowed to extract almost 80 % of the quantitative content of flavonoids from the raw material. The main technological parameters of Acorus calamus leaves were also determined. The obtained results will be the basis for the development of the technology of obtaining phyto-substance from the leaves of Acorus calamus.

Conclusions. The selection of optimal extraction conditions of biologically active compounds from Acorus calamus leaves was carried out. The obtained results show that in order to obtain extracts with the maximum content of biologically active substances (flavonoids), it is advisable to use raw materials with a particle size of 0.5 to 1.0 mm, as an extractant 70 % alcohol and an extraction time of 4 hours.

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Author Biographies

Maksym Yaremenko, National University of Pharmacy

Department of botany

Tetiana Gontova, National University of Pharmacy

Department of botany

Liudmyla Boryak, National University of Pharmacy

Department of organic chemistry

Olga Mala, National University of Pharmacy

Department of botany

Oleksiy Andryushayev, National University of Pharmacy

Department of Industrial Technology of Drugs

References

WHO (2004). WHO Guidelines on Safety Monitoring of Herbal Medicines in Pharmacovigilance Systems. Geneva: World Health Organization. Available at: http://apps.who.int/medicinedocs/documents/s7148e/s7148e.pdf

Kulbat, K. (2016). The role of phenolic compounds in plant resistance Biotechnol Food Sci, 80 (2), 97–108

Lin, D., Xiao, M., Zhao, J., Li, Z., Xing, B., Li, X. et. al. (2016). An Overview of Plant Phenolic Compounds and Their Importance in Human Nutrition and Management of Type 2 Diabetes. Molecules, 21 (10), 1374. doi: http://doi.org/10.3390/molecules21101374

State Register of Medicines of Ukraine. Available at: http://www.drlz.com.ua/ Last accessed: 19.02.2020

Wichtl, M. (2004). Herbal drugs and phytopharmaceuticals. Boca Raton: CRC Press, 115–117.

Arasan, El. R., Vijayalakshmi, M., Garikapati, D. (2009). Acorus calamus linn: Chemistry and Biology. Research Journal of Pharm. and Tech, 2 (2), 39–41.

Rajput, S. B., Tonge, M. B., Karuppayil, S. M. (2014). An overview on traditional uses and pharmacological profile of Acorus calamus Linn. (Sweet flag) and other Acorus species. Phytomedicine, 21 (3), 268–276. doi: http://doi.org/10.1016/j.phymed.2013.09.020

Balakumbahan, R., Rajamani, K., Kumanan, K. (2010). Acorus calamus: An overview. Journal of Medicinal Plants Research. 4 (25), 2740–2745.

Yadav, D., Srivastava, S., Tripathi, Y. B. (2019). Acorus Calamus A Review. International Journal of Scientific Research in Biological Sciences, 6 (4), 62–67. doi: http://doi.org/10.26438/ijsrbs/v6i4.6267

Devi, S. A., Ganjewala, D. (2011). Antioxidant Activities of Methanolic Extracts of Sweet-Flag (Acorus calamus) Leaves and Rhizomes. Journal of Herbs, Spices & Medicinal Plants, 17 (1), 1–11. doi: http://doi.org/10.1080/10496475.2010.509659

Kim, H., Han, T.-H., Lee, S.-G. (2009). Anti-inflammatory activity of a water extract of Acorus calamus L. leaves on keratinocyte HaCaT cells. Journal of Ethnopharmacology, 122 (1), 149–156. doi: http://doi.org/10.1016/j.jep.2008.12.011

Venskutonis, P. R., Dagilyte, A. (2003). Composition of Essential Oil of Sweet Flag (Acorus calamusL.) Leaves at Different Growing Phases. Journal of Essential Oil Research, 15 (5), 313–318. doi: http://doi.org/10.1080/10412905.2003.9698598

Yaremenko, M. S., Gontova, T.M., Sira, L.M. (2018). About use and identification of not officinalis raw materials – Acorus calamus L. Leaves. Medical and clinical chemistry, 20 (1), 105–110. doi: http://doi.org/10.11603/mcch.2410-681x.2018.v0.i1.8772

Yaremenko, M. S., Gontova, T. M., Kotova, E. E. (2018). Perspective for using Acorus Calamus leaves in medicine. The 9th International Conference on Pharmaceutical Sciences and Pharmacy Practice. Kaunas.

Minarchenko, V. M. (2012). Resources of medicinal plants of Ukraine: differentiation, dynamics, strategy of optimization of use and preservation. Kyiv, 36.

Industrial medicine technology. Electronic textbook (2010). Available at: http://ztl.nuph.edu.ua/medication/content.html

Yaremenko, M. S., Hontova, T. M., Kotova, E. E. (2018). Terminy zahotivli lystia lepekhy zvychainoi (Acorus Calamus L.): inform. lyst N° 367-2018. Ukrainskyi tsentr naukovoi medychnoi informatsii ta patentolitsenziinoi roboty (Ukrmedpatentinform). Kyiv, 40.

Derzhavna Farmakopeia Ukrainy. Vol. 1 (2015). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yak osti likarskykh zasobiv», 1128.

Derzhavna Farmakopeia Ukrainy. Vol. 3 (2014). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yak osti likarskykh zasobiv», 732.

Kriukova, A., Vladymyrova, I. (2018). Determination Of Technological Parameters And Indicators Of The Quality Of New Herbal Collection. EUREKA: Health Sciences, 6, 61–68. doi: http://doi.org/10.21303/2504-5679.2018.00801

Vyshnevska, L. I., Shmalko, O. O., Soldatov, D. P. (2019). Study on the development of multicomponent dry uroholum extract and its pharmaco-technological indicators. Management, Economics and Quality Assurance in Pharmacy, 2 (58), 16–21. doi: http://doi.org/10.24959/uekj.19.19

Yanickaya, A. V., Mitrofanova, I. Yu., Heilik, Yu. V. (2014). Optimizaciya processa ekstragirovaniya flavonoidov iz travy melkolepestnika kanadskogo. Vestnik Volgogradskogo gosudarstvennogo medicinskogo universiteta, 2 (50), 20–23.

Kutateladze, G. R., Fedoseeva, L. M. (2019). Research in the Development and Validation of the Method of Quantitative Determination of Flavonoids in the Common Sorrel Herba, Gathered in the Altai Territory. Drug development & registration, 8 (2), 80–86. doi: http://doi.org/10.33380/2305-2066-2019-8-2-80-86


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Published
2020-05-25
How to Cite
Yaremenko, M., Gontova, T., Boryak, L., Mala, O., & Andryushayev, O. (2020). DETERMINATION OF OPTIMAL EXTRACTION CONDITIONS OF PHENOLIC COMPOUNDS FROM ACORUS CALAMUS LEAVES. EUREKA: Health Sciences, (3), 63-70. https://doi.org/10.21303/2504-5679.2020.001317
Section
Pharmacology, Toxicology and Pharmaceutical Science