STUDY OF BONE MARROW ASPIRATE INJECTION EFFECT ON DENERVATED MUSCLE ACCORDING TO ELECTROMYOGRAPHY STUDIES
Abstract
The aim. To study in experiment the effect of bone marrow aspirate injection on the course of denervation-reinnervation processes in skeletal muscle using neurophysiological research method.
Materials and methods. Experimental study was performed on 36 mature rabbits weighing 3-4 kg. Experimental model of the peripheral nerve acute injury and neurorrhaphy is taken as a basis. Animals were divided into 4 groups of 9 animals per group (3 animals for each experimental period). At 8, 12, and 16 weeks after surgery, all rabbits underwent needle EMG of the target muscles (m. gastrocnemius, and m. tibialis anterior) to study denervation-reinnervation changes.
Results. Results of target muscles electromyographic examination are shown. As norm, we took motor unit potentials parameters, which were registered during the study of contralateral (not operated) pelvic limbs.
Largest number of registered MUPs at different stages of the denervation-reinnervation process was observed, and was significantly higher (α = 0.07) in the groups in which bone marrow aspirate was injected in relation to group without bone marrow aspirate injection. Changes in the parameters of the registered MUPs in all groups corresponded to the general characteristics of the denervation-reinnervation process.
Conclusions. Injection of bone marrow aspirate into the target muscles during surgery and in the early stages of reinnervation (in experimental study it is 7 weeks after surgery) – reliably (α = 0.07) promotes improvement of reinnervation processes in muscles, which is manifested by registration of more motor unit potentials.
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