Catalase activity as signal of antioxydant system affection under influence of limb ischemia-reperfusion

Keywords: ischemia-reperfusion, blood loss, trauma, tourniquet, catalase, experiment, liver, lipid peroxidation

Abstract

The use of hemostatic tourniquet is a proved means of primary care. However, systemic disorders, as well as ultrastructural, in the area of compression can significantly worsen the condition of the injured organism.

The aim. Estimation of catalase level in rats’ liver on the background of modifications of ischemic-reperfusion syndrome to know the severest pathogenic combination for organism.

Materials and methods. 260 white adult male rats were divided into 5 groups: control (KG), EG1 – simulation of isolated ischemia-reperfusion syndrome (IRS) of the limb, EG2 – simulation of isolated volumetric blood loss, EG3 – combination of IRS of the limb with blood loss, EG4 – simulation of isolated mechanical injury of the thigh, EG5 – combination of IRS of the limb and mechanical injury. The variability of catalase level in liver was analyzed.

Results. It was found that each of the experimental interventions has led to changes of catalase activity in the liver. The most expressed pathological expressions were observed on the 3rd after interventions, when the studied index in EG3 was lower than in EG1 and EG2 in 6,2 times and by 33,1 %. On the 7th day catalase activity in EG3 was in 9,4 times and by 44,5 % times lower than in EG1 and in EG2 data concordantly. The combination of limb ischemia-reperfusion with blood loss in EG3 led to exhausting of liver antioxydant enzyme catalase in the most critical posttraumatic period (day 3). The same, but less significant effect was registered in the group of combination of mechanical trauma with ischemia-reperfusion in EG5. This proved the role of the tourniquet as a factor that complicated the course of traumatic disease due to ischemic reperfusion.

Conclusions. In this experiment, founded risk factors of combination of ischemia-reperfusion with heavy blood loss emphasized the importance and particular attention on such widespread method of bleeding tratment, as the imposition of a tourniquet, as in our experiment it triggered risk factors of ischemia-reperfusion. It was shown katalase activity depression respectively to the periods of increasing of lipid peroxydation. There was peculiarity, that on the base of isolated IRS catalase activity was increased in 2,5 times comparely to control group, whereas the hardest depression of it was found on the background of IRS, combined with blood loss – catalase activity was lower, comparely to KG – in 2,5 times. The importance of understanding the suppression of hepatocytes’ antyoxydants is great, as it might help in prevention the development of liver failure or hepatorenal syndrome on the background of limb ischemia-reperfusion.

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Author Biography

Nataliya Volotovska, I. Horbachevskyi Ternopil National Medical University

Department of Physiology, Bioethics and Biosafety

References

Coling, D., Chen, S., Chi, L.-H., Jamesdaniel, S., Henderson, D. (2009). Age-related changes in antioxidant enzymes related to hydrogen peroxide metabolism in rat inner ear. Neuroscience Letters, 464 (1), 22–25. doi: http://doi.org/10.1016/j.neulet.2009.08.015

Davies, K. (2001). An Overview of Oxidative Stress. IUBMB Life, 50 (4), 241–244. doi: http://doi.org/10.1080/713803723

Bissinger, R., Bhuyan, A. A. M., Qadri, S. M., Lang, F. (2018). Oxidative stress, eryptosis and anemia: a pivotal mechanistic nexus in systemic diseases. The FEBS Journal, 286 (5), 826–854. doi: http://doi.org/10.1111/febs.14606

Gulek, B.; Trzcinska, M. (Ed.) (2011). Ischemia Reperfusion Injury in Kidney Transplantation. Kidney Transplantation – New Perspectives, 214–222. doi: http://doi.org/10.5772/18289

Glorieux, C., Calderon, P. B. (2017). Catalase, a remarkable enzyme: targeting the oldest antioxidant enzyme to find a new cancer treatment approach. Biological Chemistry, 398 (10), 1095–1108. doi: http://doi.org/10.1515/hsz-2017-0131

Nadeem, M. S., Khan, J. A., Murtaza, B. N., Muhammad, K., Rauf, A. (2015). Purification and Properties of Liver Catalase from Water Buffalo (Bubalus bubalis). South Asian Journal of Life Sciences, 3 (2), 51–55. doi: http://doi.org/10.14737/journal.sajls/2015/3.2.51.55

Erturk, E. (2014). Ischemia-Reperfusion Injury and Volatile Anesthetics. BioMed Research International, 2014, 1–7. doi: http://doi.org/10.1155/2014/526301

Bordakov, V. N., Alekseev, S. A., Chumanevich, O. A., Patsay, D. I., Bordakov, P. V. (2013). Crash-syndrome. Military medicine, 1, 26–32. Available at: http://rep.bsmu.by/handle/BSMU/2213

Fernández, A. R., Sánchez-Tarjuelo, R., Cravedi, P., Ochando, J., López-Hoyos, M. (2020). Review: Ischemia Reperfusion Injury – A Translational Perspective in Organ Transplantation. International Journal of Molecular Sciences, 21 (22), 8549. doi: http://doi.org/10.3390/ijms21228549

Teli, A., Ghatnatti, R. (2020). Association between blood transfusion and serum creatinine as a major risk factor in patients undergoing cardiac surgeries: An observational study. National Journal of Physiology, Pharmacy and Pharmacology, 10 (1), 59–63. doi: http://doi.org/10.5455/njppp.2020.10.1135913112019

Tsymbaliuk, H. Y. (2018). Daily urine renal state under ischemic-reperfusion syndrome of limbs, abdominal injury with hypovolemic shock and their combination in the early period of traumatic disease. Achievements of clinical and experimental medicine, 3, 163–169.

Wall, P. L., Duevel, D. C., Hassan, M. B., Welander, J. D., Sahr, S. M., Buising, C. M. (2013). Tourniquets and Occlusion: The Pressure of Design. Military Medicine, 178 (5), 578–587. doi: http://doi.org/10.7205/milmed-d-12-00490

Pro zatverdzhennia Poriadku provedennia klinichnykh vyprobuvan likarskykh zasobiv ta ekspertyzy materialiv klinichnykh vyprobuvan i Typovoho polozhennia pro komisii z pytan etyky (2009). Law of the Ministry of Health of Ukraine No. 690. 23.09.2009. Available at: https://zakon.rada.gov.ua/laws/show/z1010-09#Text

Korolyuk, V. A., Ivanova, L. I., Mayorova, I. G., Tokarev, V. E. (1988). Method for determining catalase activity. Laboratory work, 1, 16–19.

Televiak, A. T. (2018). The dynamic of indicators of lipid peroxidation and antioxidant protection in muscle tissue of the hind limbs of the rats in development of the ischemic-reperfusion syndrome (experimental study). Achievements of clinical and experimental medicine, 3, 132–139. doi: http://doi.org/10.11603/1811-2471.2018.v0.i3.9318

Volotovska, N. V., Hudyma, A. A. (2020). Features of hepatorenal reaction on the background of experimental ischemic-reperfusion syndrome. Bulletin of Problems Biology and Medicine, 2 (156), 86–91. doi: http://doi.org/10.29254/2077-4214-2020-2-156-86-91

Volotovska, N. V., Hudyma, A. A. (2020). Significance of endogenous intoxication markers in the prognosis assessment of ischemic reperfusion limb syndrome. Bulletin of medical and biological research, 3 (5), 24–31. doi: http://doi.org/10.11603/bmbr.2706-6290.2020.3.11357

Bensard, D. D., Brown, J. M., Anderson, B. O., Banerjee, A., Shanley, P. F., Grosso, M. A. et. al. (1990). Induction of endogenous tissue antioxidant enzyme activity attenuates myocardial reperfusion injury. Journal of Surgical Research, 49 (2), 126–131. doi: http://doi.org/10.1016/0022-4804(90)90250-6

Vertuani, S., Angusti, A., Manfredini, S. (2004). The Antioxidants and Pro-Antioxidants Network: An Overview. Current Pharmaceutical Design, 10 (14), 1677–1694. doi: http://doi.org/10.2174/1381612043384655

Gutierrez, G., Reines, H. D., Wulf-Gutierrez, M. (2004). Clinical review: Hemorrhagic shock. Critical care, 8 (5), 373–381. doi: http://doi.org/10.1186/cc2851

Churpek, M. M., Zadravecz, F. J., Winslow, C., Howell, M. D., Edelson, D. P. (2015). Incidence and Prognostic Value of the Systemic Inflammatory Response Syndrome and Organ Dysfunctions in Ward Patients. American Journal of Respiratory and Critical Care Medicine, 192 (8), 958–964. doi: http://doi.org/10.1164/rccm.201502-0275oc

NeSmith, E. G., Weinrich, S. P., Andrews, J. O., Medeiros, R. S., Hawkins, M. L., Weinrich, M. C. (2011). Demographic Differences in Systemic Inflammatory Response Syndrome Score After Trauma. American Journal of Critical Care, 21 (1), 35–41. doi: http://doi.org/10.4037/ajcc2012852

Knafl, E. G., Hughes, J. A., Dimeski, G., Eley, R. (2018). Rhabdomyolysis: Patterns, Circumstances, and Outcomes of Patients Presenting to the Emergency Department. Ochsner Journal, 18 (3), 215–221. doi: http://doi.org/10.31486/toj.17.0112

Park, J.-S., Seo, M.-S., Gil, H.-W., Yang, J.-O., Lee, E.-Y., Hong, S.-Y. (2013). Incidence, Etiology, and Outcomes of Rhabdomyolysis in a Single Tertiary Referral Center. Journal of Korean Medical Science, 28 (8), 1194–1199. doi: http://doi.org/10.3346/jkms.2013.28.8.1194

Candela, N., Silva, S., Georges, B., Cartery, C., Robert, T. et. al. (2020). Short- and long-term renal outcomes following severe rhabdomyolysis: a French multicenter retrospective study of 387 patients. Annals of Intensive Care, 10 (1). doi: http://doi.org/10.1186/s13613-020-0645-1

Pianta, T. J., Succar, L., Davidson, T., Buckley, N. A., Endre, Z. H. (2017). Monitoring treatment of acute kidney injury with damage biomarkers. Toxicology Letters, 268, 63–70. doi: http://doi.org/10.1016/j.toxlet.2017.01.001


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Published
2021-03-31
How to Cite
Volotovska, N. (2021). Catalase activity as signal of antioxydant system affection under influence of limb ischemia-reperfusion. EUREKA: Health Sciences, (2), 24-30. https://doi.org/10.21303/2504-5679.2021.001648
Section
Medicine and Dentistry