STANDARDIZATION OF EUCALYPTUS GLOBULUS LEAVES AND CETRARIA ISLANDICA SLAN
The aim. The aim of this study was to propose methods of standardization of Eucalyptus globulus leaves and Cetraria islandica slan by the main group of biologically active substances (BAS). This was done for further development of complex medicines based on Eucalyptus globulus leaves and Cetraria islandica slan.
These studies provide an opportunity for further development and standardization of extracts from these plants, and the main aim of this study is the development and standardization of a complex medicine.
Materials and methods. Thin layer chromatography was used to determine the presence of these markers. Quantitative values of these biologically active substances were determined by spectrophotometric method according to the relevant methods of the State Pharmacopoeia of Ukraine.
Results. Markers for the certification of Cetraria islandica and Eucalyptus globulus have been proposed in this study. Chlorophylls and 1,8-cineole were chosen as a marker for the study of Eucalyptus globulus, and polysaccharides were chosen as a marker for Cetraria islandica. The qualitative and quantitative content of these markers in the studied objects was studied.
Further research in this direction will be aimed at developing methods for control and standardization of water and alcohol extracts from these plants.
Conclusions. Biologically active substances in medicinal plant raw materials were studied. It is proposed to study extracts and finished products from Eucalyptus globulus leaves and Cetraria islandica slan using these methods.
The method of qualitative and quantitative determination of polysaccharides in Cetraria islandica slan is easy to apply and easily reproducible. It is important to be able to standardize Eucalyptus globulus leaves for essential oils and chlorophylls.
The biologically active substances-markers found in Eucalyptus globulus leaves and Cetraria islandica slan will be used for the development of herbal medicines, the standardization of which will be carried out for the same compounds.
Osawa, K., Yasuda, H., Morita, H., Takeya, K., Itokawa, H. (1995). Eucalyptone from Eucalyptus globulus. Phytochemistry, 40 (1), 183–184. doi: http://doi.org/10.1016/0031-9422(95)00233-w
Katalinic, V., Milos, M., Kulisic, T., Jukic, M. (2006). Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chemistry, 94 (4), 550–557. doi: http://doi.org/10.1016/j.foodchem.2004.12.004
Bailey, C. (2008). Metformin: effects on micro and macrovascular complications in type 2 diabetes. Cardiovascular Drugs and Therapy, 22 (3), 215–224. doi: http://doi.org/10.1007/s10557-008-6092-0
Kim, J.-P., Lee, I.-K., Yun, B.-S., Chung, S.-H., Shim, G.-S., Koshino, H., Yoo, I.-D. (2001). Ellagic acid rhamnosides from the stem bark of Eucalyptus globulus. Phytochemistry, 57 (4), 587–591. doi: http://doi.org/10.1016/s0031-9422(01)00146-7
Koshovyy, O., Komissarenko, A., Kovaleva, A., Mudryk, I. (2005). Microelement, amino acid and polysaccharide composition of eucalyptus leaves. Phytotherapy, 3, 59–62.
State Pharmacopoeia of Ukraine. Vol. 2 (2018). Kharkiv: State Enterprise "Ukrainian Scientific Pharmacopoeial Center for Drug Quality", 416. Available at: http://sphu.org/wp-content/uploads/2018/07/Gfu2.3.pdf
Guidelines of the Ministry of Health of Ukraine "Drugs. Good laboratory practice: ST-N MOH 42-6.0: 2008 (2012). Kyiv, 27. Available at: https://compendium.com.ua/uk/clinical-guidelines-uk/standartizatsiya-farmatsevtichnoyi-produktsiyi-tom-2/st-n-mozu-42-6-0-2008/
Pavlyuk, I., Stadnytska, N., Jasicka-Misiak, I., Wieczorek, P., Zagoriy, G., Brezvyn, O. et. al. (2015). Study of biological activity of secondary extract from oregano herbs waste qoriganum vulgaret. Ukrainian Biopharmaceutical Journal, 1 (36), 21–24. Available at: http://22.214.171.124/bitstream/123456789/7389/1/UBFZh1-15-3.pdf
Kovalev, V., Marchyshyn, S., Hvorost, O. et. al.; Kovalev, V., Marchyshyn, S., Hvorost, O., Isakova, T. (Eds.) (2014). Workshop on the identification of medicinal plant raw materials. Ternopil: TDMU, 264. Available at: https://dspace.nuph.edu.ua/handle/123456789/21239
Assunção, M. F. G., Amaral, R., Martins, C. B., Ferreira, J. D., Ressurreição, S., Santos, S. D. et. al. (2016). Screening microalgae as potential sources of antioxidants. Journal of Applied Phycology, 29 (2), 865–877. doi: http://doi.org/10.1007/s10811-016-0980-7
Grodzinskiy, A. (1990). Medicinal Plants: Encyclopedic Handbook. Kyiv: Heads edit URE.
Diakon, I. V., Stadnytska, N. E., Hubytska, I. I., Lylo, V. V., Petrykevych, V. R. (2017). Using of iceland moss in treatment of infectiоus respiratory diseases and prospects of creating new drugs based on it. Visnyk Natsionalnoho universytetu "Lvivska politekhnika". Serie: Khimiia, tekhnolohiia rechovyn ta yikh zastosuvannia, 868, 234–241. Available at: http://ena.lp.edu.ua:8080/handle/ntb/40646
Assessment report on Eucalyptus globulus Labill., folium (2012). The European Medicines Agency (EMA). Available at: https://www.ema.europa.eu/en/documents/herbal-report/draft-assessment-report-eucalyptus-globulus-labill-folium_en.pdf
Huneck, S., Yoshimura, I. (1996). Identification of Lichen Substances. Springer, 11–123. doi: http://doi.org/10.1007/978-3-642-85243-5_2
Marxen, K., Vanselow, K., Lippemeier, S., Hintze, R., Ruser, A., Hansen, U.-P. (2007). Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements. Sensors, 7 (10), 2080–2095. doi: http://doi.org/10.3390/s7102080
Basaraba, R. (2019). Pharmacognosy study of common pussytoes (Antennaria dioica) in order to develop new medicines. Lviv, 184. Available at: http://nauka.meduniv.lviv.ua/wp-content/uploads/2019/10/ %D0 %94 %D0 %B8 %D1 %81.- %D0 %91 %D0 %B0 %D1 %81 %D0 %B0 %D1 %80 %D0 %B0 %D0 %B1 %D0 %B0.pdf
Hardel, D. K., Sahoo, L. (2011). A review on phytochemical and pharmacological of Eucalyptus globulus: a multipurpose tree. International Journal of Research in Ayurveda and Pharmacy, 32, 1527–1530. Available at: http://www.ijrap.net/admin/php/uploads/648_pdf.pdf
Renedof, O., Otero, J., Mira, J. (2000). Oile essentielle de Eucalyptus globulus L. de Contabrie. Variation fn cours de la distillation. Plant. med. et phetother, 1 (24), 31–38.
Martines, M. (2006). Estudio preliminar de los aceites esenciales de Evcalyptus introducides en la region de Topes de Callantes. Rev. cub. Farm, 2, 159–168.
Chueshov, V., Gladui, E., Sayyko, V. et. al. (2013). Technology of medicines for industrial production. Part 1. Kharkiv: NFaU, 694. Available at: https://dspace.nuph.edu.ua/handle/123456789/23564
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