Free radical damage: role in varicella zoster virus infection

Keywords: chickenpox, lipid peroxidation, antioxidant system, glutathione system

Abstract

Study of the role of lipid peroxidation and antioxidant system in patients with infectious diseases is of great interest to researchers. Numerous studies have led to a common understanding of their contribution to the pathogenesis of infectious diseases, as well as to the complications development. However, the state of lipid peroxidation and antioxidant protection in patients with chickenpox (varicella) has not been sufficiently studied which is important for the development of new targeted treatments.

The aim of the research - to assess the state of lipid peroxidation (LPO) and the antioxidant system (AOS) in the dynamics in patients with chickenpox, depending on the severity of the disease.

Materials and methods. We selected for participating in the study 240 chickenpox patients (142 women and 98 men) aged 18-40 years. Chickenpox was diagnosed using clinical, serological, and molecular genetic methods. The state assessment of LPO in chickenpox patients was based on the determination of serum diene conjugates (DC), malondialdehyde (MDA) levels and the total oxidative activity (TOA) of blood plasma.

Results. Study showed that the process of lipid peroxidation increases and the activity of the antioxidant system decreases in patients with chickenpox during the acute period of the disease. The extent of these processes becomes greater as the severity of chickenpox increases. A decrease in the activity of antioxidant enzymes and a disruption in the functioning of the glutathione system in patients with moderate to severe hypertension leads to disruption of protective mechanisms and, as a result, to an increase in the free radical chain reactions, the uncontrolled growth of which causes irreversible damage to the membranes of various cells, which underlies visceropathy in chickenpox patients.

Conclusions. Antioxidant system is activated in the acute period in patients with mild chickenpox. It is evidenced by an increase in the overall antioxidant activity of blood plasma (p=0.045) and red blood cells (p=0.00087), in the activity of catalase (p=0.001), superoxide dismutase (p=0.0093), glutathione peroxidase (p=0.036), glutathione reductase of plasma and red blood cells, and an increase in the concentration of oxidized and reduced glutathione in blood. Lipid peroxidation is hyperactivated in the acute period in patients with a moderate and severe course of the disease and an excess amount of primary and secondary hydroperoxides of fatty acids accumulates in the blood. This is accompanied by indicates a developing imbalance between the oxidative and antioxidant systems in group of patients with moderate and severe chickenpox. Our findings confirm the usefulness of antioxidants in the treatment of chickenpox.

Downloads

Download data is not yet available.

Author Biographies

Olga Volobuieva, V. N. Karazin Kharkiv National University

Department of Infectious Diseases and Clinical Immunology, School of Medicine

Tetiana Liadova, V. N. Karazin Kharkiv National University

Department of Infectious Diseases and Clinical Immunology, School of Medicine

Mykola Popov, V. N. Karazin Kharkiv National University

Department of Infectious Diseases and Clinical Immunology, School of Medicine

Olga Sorokina, V. N. Karazin Kharkiv National University

Department of Infectious Diseases and Clinical Immunology, School of Medicine

Olena Ognivenko, V. N. Karazin Kharkiv National University

Department of Infectious Diseases and Clinical Immunology, School of Medicine

Diana Dorosh, V. N. Karazin Kharkiv National University

Department of Infectious Diseases and Clinical Immunology, School of Medicine

References

Schürmann, N., Forrer, P., Casse, O., Li, J., Felmy, B., Burgener, A.-V. et. al. (2017). Myeloperoxidase targets oxidative host attacks to Salmonella and prevents collateral tissue damage. Nature Microbiology, 2 (4). doi: http://doi.org/10.1038/nmicrobiol.2016.268
Alavian, S. M., Showraki, A. (2016). Hepatitis B and its Relationship With Oxidative Stress. Hepatitis Monthly, 16 (9). doi: http://doi.org/10.5812/hepatmon.37973
Darenskaya, M. A., Grebenkina, L. A., Sholokhov, L. F., Rashidova, M. A., Semenova, N. V., Kolesnikov, S. I., Kolesnikova, L. I. (2016). Lipid Peroxidation Activity in Women with Chronic Viral Hepatitis. Free Radical Biology and Medicine, 100, S192. doi: http://doi.org/10.1016/j.freeradbiomed.2016.10.525
Isberg, R. R. (2017). Internalization of Microbial Pathogens by Integrin Receptors and the Binding of the Yersinia pseudotuberculosis Invasin Protein. Integrins – The Biological Problems. CRC Press, 197–216.
Emene, P. E., Kravchenko, I. E., Aibatova, G. I., Rizvanov, A. A. (2015). Antioxidant system gene polymorphism in patients with erysipelas and their role in the development of the disease. Genes and cells, 10 (4), 118–122.
Mayer, K., Mundigl, O., Kettenberger, H., Birzele, F., Stahl, S., Pastan, I., Brinkmann, U. (2019). Diphthamide affects selenoprotein expression: Diphthamide deficiency reduces selenocysteine incorporation, decreases selenite sensitivity and pre-disposes to oxidative stress. Redox Biology, 20, 146–156. doi: http://doi.org/10.1016/j.redox.2018.09.015
Shustval, M. F., Lyadova, T. I., Volobueva, O. V., Pavlikova, K. V., Gamilovskaya, A. P. (2018). State of lipid peroxidation and oxidant system in patients with infectious mononucleosis. Bulletin of the VN Kharkiv National University Karazin. Series "Medicine", 36, 33–40. doi: http://doi.org/10.26565/2313-6693-2018-36-05
Bwititi, P., Chinkwo, K. (2016). Oxidative stress markers in infectious respiratory diseases: current clinical practice. International Journal of Research in Medical Sciences, 1802–1813. doi: http://doi.org/10.18203/2320-6012.ijrms20161727
Ramana, K. V., Srivastava, S., Singhal, S. S. (2017). Lipid Peroxidation Products in Human Health and Disease 2016. Oxidative Medicine and Cellular Longevity, 2017, 1–2. doi: http://doi.org/10.1155/2017/2163285
Tuktanov, N. V., Kichigin, V. A. (2013). Kichigin features lipid peroxidation of thyroid dysfunction. Bulletin of the Chuvash University, 3, 55–560.
Karpishchenko, L. I. (2002). Reference book. Medical laboratory equipment. Saint Petersburg: Intermedika, 245.
Kolesnikova, L. I., Darenskaya, M. A., Rashidova, M. A., Sholokhov, L. F., Grebenkina, L. A., Vanteeva, O. A. (2016). Lipid Peroxidation State in Women of Reproductive Age with Acute Form of Viral Hepatitis. Annals of the Russian Academy of Medical Sciences, 71 (1), 11–15. doi: http://doi.org/10.15690/vramn525
Putilina, F. E.; Prokhorova, M. I. (Ed.) (1982). Determination of glutathione reductase activity. Methods of biochemical research, 181–186.
Poprac, P., Jomova, K., Simunkova, M., Kollar, V., Rhodes, C. J., Valko, M. (2017). Targeting Free Radicals in Oxidative Stress-Related Human Diseases. Trends in Pharmacological Sciences, 38 (7), 592–607. doi: http://doi.org/10.1016/j.tips.2017.04.005
Polozova, E. I., Trokhina, I. E., Kurkina, N. V., Gorshenina, E. I. (2017). Estimation of efficiency of application of combined therapy in complex treatment of duodenal ulcer. Modern problems of science and education, 3, 67.
Saveris, M. J., Mayakova, E. I. (2018). Estimation of efficiency of application of combined therapy in complex treatment of duodenal ulcer. Tomorrow's Medicine, 43–44.
Gaschler, M. M., Stockwell, B. R. (2017). Lipid peroxidation in cell death. Biochemical and Biophysical Research Communications, 482 (3), 419–425. doi: http://doi.org/10.1016/j.bbrc.2016.10.086
Pawluk, H., Pawluk, R., Robaczewska, J., Kędziora-Kornatowska, K., Kędziora, J. (2017). Biomarkers of antioxidant status and lipid peroxidation in elderly patients with hypertension. Redox Report, 22 (6), 542–546. doi: http://doi.org/10.1080/13510002.2017.1372072
Bhattacharya, S. (2015). Reactive oxygen species and cellular defense system. Free radicals in human health and disease. New Delhi: Springer, 17–29. doi: http://doi.org/10.1007/978-81-322-2035-0_2
Conti, V., Corbi, G., Simeon, V., Russomanno, G., Manzo, V., Ferrara, N., Filippelli, A. (2015). Aging-related changes in oxidative stress response of human endothelial cells. Aging Clinical and Experimental Research, 27 (4), 547–553. doi: http://doi.org/10.1007/s40520-015-0357-9
Dua, A., Kaur, N., Gupta, P., Mittall, A., Gupta, S. K. (2017). Oxidative stress induced cell damage and antioxidant enzyme response in human lymphocytes. International Journal of Pharmaceutical & Biological Archive, 8, 33–39.
Ighodaro, O. M., Akinloye, O. A. (2018). First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexandria Journal of Medicine, 54 (4), 287–293. doi: http://doi.org/10.1016/j.ajme.2017.09.001
Allocati, N., Masulli, M., Di Ilio, C., Federici, L. (2018). Glutathione transferases: substrates, inihibitors and pro-drugs in cancer and neurodegenerative diseases. Oncogenesis, 7 (1). doi: http://doi.org/10.1038/s41389-017-0025-3
Mead, J. F., Stein, R. A., Wu, G. S. (2019). Metabolic Fate of Peroxidation Products. Cellular Antioxidant Defense Mechanisms. CRC Press, 89–102. doi: http://doi.org/10.1201/9780429289286-6
Griffiths, H. R., Gao, D., Pararasa, C. (2017). Redox regulation in metabolic programming and inflammation. Redox Biology, 12, 50–57. doi: http://doi.org/10.1016/j.redox.2017.01.023
Sies, H. (2017). Hydrogen peroxide as a central redox signaling molecule in physiological oxidative stress: Oxidative eustress. Redox Biology, 11, 613–619. doi: http://doi.org/10.1016/j.redox.2016.12.035
Sies, H., Berndt, C., Jones, D. P. (2017). Oxidative Stress. Annual Review of Biochemistry, 86 (1), 715–748. doi: http://doi.org/10.1146/annurev-biochem-061516-045037
Sies, H. (2015). Oxidative stress: a concept in redox biology and medicine. Redox Biology, 4, 180–183. doi: http://doi.org/10.1016/j.redox.2015.01.002
Nasiri, S., Hedayati, M., Riahi, S., Robati, R., Khazan, M. (2018). Elevated serum nitric oxide and hydrogen peroxide levels as potential valuable predictors of herpes zoster. Asian Pacific Journal of Tropical Medicine, 11 (6), 381. doi: http://doi.org/10.4103/1995-7645.234766
Muriel, P. (2017). Peroxidation of lipids and liver damage. Oxidants, antioxidants and free radicals. CRC Press, 237–257. doi: http://doi.org/10.1201/9780203744673-14
Georgieva, A., Vilhelmova, N., Muckova, L., Tzvetanova, E., Alexandrova, A., Mileva, M. (2017). Alterations in oxidative stress parameters in MDBK cells, infected by herpes simplex virus-1. Comptes rendus de l’Académie bulgare des Sciences, 70 (5).

👁 38
⬇ 39
Published
2021-07-30
How to Cite
Volobuieva, O., Liadova, T., Popov, M., Sorokina, O., Ognivenko, O., & Dorosh, D. (2021). Free radical damage: role in varicella zoster virus infection. EUREKA: Health Sciences, (4), 41-47. https://doi.org/10.21303/2504-5679.2021.001990
Section
Medicine and Dentistry