• Anna Kriukova National University of Pharmacy; State Scientific-Research Laboratory of Nuph For Medicinal Substances Quality Control
  • Inna Vladymyrova National University of Pharmacy
Keywords: rheumatology, herbal collection, Harpagophytum procumbens, standardization


Rheumatic diseases include more than 1000 nosological forms, among which the highest incidence is osteoarthritis, rheumatoid arthritis and osteoporosis. The predominantly advanced age, the presence of comorbid diseases, the duration of the course of the disease and the need for concomitant treatment complicate the conduct of anti-inflammatory and analgesic therapy in such patients. Herbal medicines, in particular in the form of doses, due to the mildness of the action and the absence of significant side effects, can be used in the complex therapy of the acute period of rheumatic diseases, as well as for the prevention of relapse.

The aim of the presented work was to develop the composition, technology and determination of the quality indices of herbal collection, which reveals analgesic and anti-inflammatory properties and can be used for the prophylaxis and auxiliary therapy of a number of conditions in rheumatology.

Materials and methods. In the course of research, the methods used in the State Pharmacopoeia of Ukraine 2.0 (SPHU) were used.

Results. As a result of the analysis of literary data and our own experimental studies on the chemical composition and pharmacological activity of certain types of medicinal plant material, we have developed herbal collection that is recommended for the treatment and prevention of rheumatic diseases of the joints. Studies have been conducted on the development of quality collection indicators, which guarantee the effectiveness, safety, reproducibility of the imposed collection. The basic technological parameters of the raw material are determined: specific, bulk and volume mass, porosity, degree, degree of shredding. In order to prevent uneven mixing, an optimal degree of grinding of the raw material of the investigated collection, which ranges from 0.20 mm to 0.35 mm, is established, which allows obtaining a homogeneous mixture and contributes to the maximum yield of extractive and active substances. A series of experimental works aimed at studying the influence of the method of infusion (for 15, 30, 45, 60 minutes and until complete cooling) on the quality of the collected water extracts from the collection was conducted. The optimal method for setting the collection for 60 minutes is the best, since the content of extractives and flavonoids in the aqueous extract was highest and amounted to 49.57±0.52 % and 0.52±0.03 % respectively.

Conclusions. The composition of the herbal drug was developed: Harpagophyti radiх, Sophoraе alabastra, Valvae fructus Phaseoli vulgaris, Polygoni avicularis herba with the following ratio of components 50: 20: 20:10. The technological parameters of the raw material are studied: specific, volumetric and bulk mass, porosity, singularity of raw materials. The optimum degree of shredding of raw material is established, which helps to maximally remove biologically active substances and extractives. Experimental way is determined the optimal mode of collection, which can be recommended to the consumer to obtain the maximum therapeutic effect.


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Author Biographies

Anna Kriukova, National University of Pharmacy; State Scientific-Research Laboratory of Nuph For Medicinal Substances Quality Control
Department of Pharmacognosy
Inna Vladymyrova, National University of Pharmacy

Scientific Methodological (Scientific Research) Laboratory on Pharmaceutical Education

Department of pharmacognosy


Ash, Z., Gaujoux-Viala, C., Gossec, L., Hensor, E. M., FitzGerald, O., Winthrop, K. et. al. (2011). A systematic literature review of drug therapies for the treatment of psoriatic arthritis: current evidence and meta-analysis informing the EULAR recommendations for the management of psoriatic arthritis. Annals of the Rheumatic Diseases, 71 (3), 319–326. doi:

Cameron, M., Chrubasik, S. (2014). Oral herbal therapies for treating osteoarthritis. Cochrane Database of Systematic Reviews. doi:

Fernandes, L., Hagen, K. B., Bijlsma, J. W. J., Andreassen, O., Christensen, P., Conaghan, P. G. et. al. (2013). EULAR recommendations for the non-pharmacological core management of hip and knee osteoarthritis. Annals of the Rheumatic Diseases, 72 (7), 1125–1135. doi:

Baloglu, H., Askin, A. (2015). Determination of the factors that affect healthrelated quality of life in patients with rheumatoid arthritis. Acta Medica Mediterranea, 31, 687–695.

Patil, P. S., Rajani, S. (2010). An advancement of analytical techniques in herbal research. International Journal of Advanced Science and Research, 1 (1), 8–14.

Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K., Latha, L. (2010). Extraction, Isolation And Characterization Of Bioactive Compounds From Plants’ Extracts. African Journal of Traditional, Complementary and Alternative Medicines, 8 (1). doi:

Santos, D. T., Veggi, P. C., Meireles, M. A. A. (2012). Optimization and economic evaluation of pressurized liquid extraction of phenolic compounds from jabuticaba skins. Journal of Food Engineering, 108 (3), 444–452. doi:

Anokwuru, C. P., Anyasor, G. N., Ajibaye, O., Fakoya, O., Okebugwu, P. (2011). Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three nigerian medicinal plants. Natural Science, 9, 53–61.

Altemimi, A., Watson, D. G., Kinsel, M., Lightfoot, D. A. (2015). Simultaneous extraction, optimization, and analysis of flavonoids and polyphenols from peach and pumpkin extracts using a TLC-densitometric method. Chemistry Central Journal, 9 (1). doi:

Mediani, A., Abas, F., Khatib, A., Tan, C. (2013). Cosmos Caudatus as a Potential Source of Polyphenolic Compounds: Optimisation of Oven Drying Conditions and Characterisation of Its Functional Properties. Molecules, 18 (9), 10452–10464. doi:

Derzhavna Farmakopeia Ukrainy. Vol. 1 (2015). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yak osti likarskykh zasobiv», 1128.

Derzhavna Farmakopeia Ukrainy. Vol. 3 (2014). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yak osti likarskykh zasobiv», 732.

Inaba, K., Murata, K., Naruto, S., Matsuda, H. (2010). Inhibitory effects of devil’s claw (secondary root of Harpagophytum procumbens) extract and harpagoside on cytokine production in mouse macrophages. Journal of Natural Medicines, 64 (2), 219–222. doi:

Fiebich, B. L., Munoz, E., Rose, T., Weiss, G., McGregor, G. P. (2012). Molecular targets of the antiinflammatory Harpagophytum procumbens (devil’s claw): inhibition of TNFα and COX-2 gene expression by preventing activation of AP-1. Phytotherapy Research, 26 (6), 806–811. doi:

Xie, Z., Lam, S., Wu, J., Yang, D., Xu, X. (2014). Chemical fingerprint and simultaneous determination of flavonoids in Flos Sophorae Immaturus by HPLC-DAD and HPLC-DAD-ESI-MS/MS combined with chemometrics analysis. Anal. Methods, 6 (12), 4328–4335. doi:

He, X., Bai, Y., Zhao, Z., Wang, X., Fang, J., Huang, L. et. al. (2016). Local and traditional uses, phytochemistry, and pharmacology of Sophora japonica L.: A review. Journal of Ethnopharmacology, 187, 160–182. doi:

Onyilagha, J. C., Islam, S. (2009). Flavonoids and other polyphenols of the cultivated species of the genus Phaseolus. International Journal of Agriculture and Biology, 11, 231–234.

Helmstädter, A. (2010). Beans and Diabetes: Phaseolus vulgaris Preparations as Antihyperglycemic Agents. Journal of Medicinal Food, 13 (2), 251–254. doi:

Carai, M. (2009). Potential efficacy of preparations derived from Phaseolus vulgaris in the control of appetite, energy intake, and carbohydrate metabolism. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 2, 145–153. doi:

Jiao, L., Yan, C., Zhang, K., Xie, J., Zhang, Y., Wen, Z. (2018). Comprehensive determination of nine polyphenols in Polygoni Avicularis Herba with a new HPLC–DAD method and their correlation with the antioxidant activities. Journal of Food Measurement and Characterization, 12 (3), 1593–1600. doi:

Piminov, A. F. Beztsennaya, T. S., Shul'ga, L. I. (2014). Izuchenie vliyaniya farmatsevticheskikh faktorov na poluchenie vodnogo izvlecheniya iz lekarstvennogo rastitel'nogo sbora. Vestnik farmatsii, 1 (63), 27–32.

Piskovatskyi, Yu. H., Vyshnevska, L. I., Heornhiiants, V. A. (2008). Rozrobka tekhnolohii likarskoho zboru «Bronkhofit». Medychna khimiia, 10 (1), 63–66.

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Pharmacology, Toxicology and Pharmaceutical Science