VILLOUS LYMPHOCYTES IN BLOOD AND BONE MARROW IN SOME FORMS OF B-CELL LYMPHOID MALIGNANCIES
Аннотация
The cytological and immunocytochemical features of the lymphocytes with villous morphology in peripheral blood and bone marrow in some B-lymphoproliferative disorders were studied. The diagnosis of hairy cell leukemia, a hairy cell leukemia variant, splenic marginal zone lymphoma and splenic diffuse red pulp small B-cell lymphoma was ascertained in accordance with the new revision of the WHO classification (2016). The neoplastic cells of hairy cell leukemia were determined by the presence of high tartrate resistant acid phosphatase (TRAP) activity. Cell surface expression of CD19, CD20 and CD21 antigens was detected. Also, the expression of CD25, CD103 and CD200, and in some cases cyclin D1, was found out. CD5, CD10 and CD23 were not detected. The immunophenotype of cells in splenic marginal zone lymphoma with villous processes also corresponded to the mature B cells. The expression of CD19, CD20 and CD21 was observed in all cases, CD11c – in 50% of patients, CD25 or CD5 – in 10% of patients. In 80% of patients, the pathologic cells did not show TRAP activity. In the bone marrow and peripheral blood cells of patients with diffuse red pulp lymphoma, TRAP activity was not detected. An immunophenotype in the hairy cell leukemia variant was different from those of classic HCL (CD19+CD20+CD22+CD103+CD11c+CD5–CD10–CD23–). Characterized immunophenotypical markers, which have differential diagnostic values in several forms of lymphoid tumors of B cell origin, will be important for the choice of treatment methods and prognosis
Скачивания
Литература
Feller, A. C., Diebold, J. (2004). Histopathology of Nodal and Extranodal Non-Hodgkin’s Lymphomas. Springer. doi: https://doi.org/10.1007/978-3-642-18653-0
Jaffe, E. S., Harris, N. L., Stein, H., Isaacson, P. G. (2008). Classification of lymphoid neoplasms: the microscope as a tool for disease discovery. Blood, 112 (12), 4384–4399. doi: https://doi.org/10.1182/blood-2008-07-077982
Swerdlow, S. H. (2008). WHO Classification of Tumors of Haematopoietic and Lymphoid Tissues. Lyon: IARC Press.
Bain, B. J. (2017). Leukaemia diagnosis. John Wiley & Sons. doi: https://doi.org/10.1002/9781119210511
Swerdlow, S. H., Campo, E., Pileri, S. A., Harris, N. L., Stein, H., Siebert, R. et. al. (2016). The 2016 revision of the World Health Organization classification of lymphoid neoplasms. Blood, 127 (20), 2375–2390. doi: https://doi.org/10.1182/blood-2016-01-643569
Canellos, G. P., Lister, T. A., Young, B. (Eds.) (2006). The Lymphomas. Elsevier, 600. doi: https://doi.org/10.1016/b978-0-7216-0081-9.x5001-8
Isaacson, P., Matutes, E., Burke, M., Catovsky, D. (1994). The histopathology of splenic lymphoma with villous lymphocytes. Blood, 84 (11), 3828–3834. doi: https://doi.org/10.1182/blood.v84.11.3828.bloodjournal84113828
Sharpe, R. W., Bethel, K. J. (2006). Hairy Cell Leukemia: Diagnostic Pathology. Hematology/Oncology Clinics of North America, 20 (5), 1023–1049. doi: https://doi.org/10.1016/j.hoc.2006.06.010
Matutes, E., Oscier, D., Montalban, C., Berger, F., Callet-Bauchu, E., Dogan, A. et. al. (2007). Splenic marginal zone lymphoma proposals for a revision of diagnostic, staging and therapeutic criteria. Leukemia, 22 (3), 487–495. doi: https://doi.org/10.1038/sj.leu.2405068
Gluzman, D. F., Sklyarenko, L. M., Nadgornaya, V. A., Kryachok, I. A. (2003). Diagnosticheskaya immunotsitohimiya opuholey. Kyiv: Morion, 156.
Copyright (c) 2020 Alyona Polishchuk, Michael Zavelevich, Daniil Gluzman
Это произведение доступно по лицензии Creative Commons «Attribution» («Атрибуция») 4.0 Всемирная.
