Apparent prevalence of brucellosis, Q-fever and toxoplasmosis in aborted goat’s at North Shoa, Ethiopia

Keywords: Abortion, Antibodies, Brucellosis, Ethiopia, Goat, North Shoa, Q-fever, Parities, Seroprevalence, Toxoplasmosis


Abortion imposes great economical loss in productivity and by product of small ruminants. The present study was conducted to determine the rate of abortion and apparent prevalence of Brucellosis, Toxoplasmosis and Q-fever in aborted goats from June 2015 to August 2019 in North Shoa, Ethiopia. During consecutive years of clinical case study 503 does were entered to mating, of which100 (19.5 %) animals aborted. There were significant differences (p=0.013) in abortion among parities. A total of 35 serum samples were collected from aborted does within six months periods. All samples were screened initially with Rose Bengal plate test (RBPT) for Brucellosis. All RBPT positive were further tested by i-ELISA. Also, serums were tasted to screen specific antibody against Q-fever and Toxoplasmosis using i-ELISA. Of total tested 64.7 % and 8.6 % of them were positive for Q-fever and Toxoplasmosis, respectively, but neither of them was positive for Brucellosis. The present clinical study revealed that abortion was the cause of kids’ loss and serological investigation of antibody against Q-fever and Toxoplasmosis showed that the agents were the major causes of abortion. Even though there was no positive reactor does to brucellosis, the result must be interpreted with care since absence of evidence is not evidence of absence. Beside of this, some samples collected from active cases were sero-negative for neither of tested antibodies; it indicated there was other cause/s of abortion in the study site. These interesting findings deserve further detail study by using more sensitive diagnostic test in order to examine the full extent of the problem in small ruminant populations. Also, an awareness-raising campaign should be launched to educate farm workers and professionals about proper preventive and control measures for such zoonotic diseases


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Author Biographies

Enyiew Alemnew Alamerew, Debre Birhan Agricultural Research Centre

Livestock Research Directorate

Erdachew Yitagesu, Debre Birhan Agricultural Research Centre

Livestock Research Directorate

Alemnew Areaya, Debre Birhan Agricultural Research Centre

Livestock Research Directorate

Derib Aydefruhim, Debre Birhan Agricultural Research Centre

Livestock Research Directorate


Agricultural sample survey. Vol. II. Report on livestock and livestock characteristics (private peasant holdings). Addis Ababa: Central Statistical Authority. Available at:

Mohammed, M., Mindaye, S., Hailemariam, Z., Tamerat, N., Muktar, Y. (2017). Sero-Prevalence of Small Ruminant Brucellosis in Three Selected Districts of Somali Region, Eastern Ethiopia. Journal of Veterinary Science and Animal Husbandry, 5 (1). doi:

The center for food security and public health. Available at:

Ali, S., Zhao, Z., Zhen, G., Kang, J. Z., Yi, Z. P. (2019). Reproductive problems in small ruminants (Sheep and goats): a substantial economic loss in the world. Large Animal Review, 25, 215–223. Available at:

Major animal production constraints in sub-Saharan Africa countries (1994). Peters.

Webster, J. P. (2010). Dubey, J.P. Toxoplasmosis of Animals and Humans. Parasites & Vectors, 3 (1). doi:

Szeredi, L., Jánosi, Sz., Tenk, M., Tekes, L., Bozsó, M., Deim, Z., Molnár, T. (2006). Epidemiological and pathological study on the causes of abortion in sheep and goats in Hungary (1998-2005). Acta Veterinaria Hungarica, 54 (4), 503–515. doi:

Tenter, A. M., Heckeroth, A. R., Weiss, L. M. (2000). Toxoplasma gondii: from animals to humans. International Journal for Parasitology, 30 (12-13), 1217–1258. doi:

Jithendran, K. P. (2004). Seroprevalence of Toxoplasma antibodies in domestic animals- an indicator of Toxoplasma gondii in the environment and human. ENVIS Bulletin Himalayan Ecology, 12 (1), 41–43. Available at:

Radostits, O. M., Gay, C. C., Hinchcliff, K. W., Constable, P. D. (2006). Veterinary medicine: a textbook of the diseases of cattle, horses, sheep, pigs and goats. London: Sounders.

Demissie, T., Tilahun, G. (2002). Study on toxoplasmosis in sheep and goats in Debre Birhan and surrounding areas in Ethiopia. Bulletin of Animal Health and Production in Africa, 49, 138–146.

Zewdu, E., Agonafir, A., Tessema, T. S., Tilahun, G., Medhin, G., Vitale, M. et. al. (2013). Seroepidemiological study of caprine toxoplasmosis in East and West Shewa Zones, Oromia Regional State, Central Ethiopia. Research in Veterinary Science, 94 (1), 43–48. doi:

Gebremedhin, E. Z., Abdurahaman, M., Hadush, T., Tessema, T. S. (2014). Seroprevalence and risk factors of Toxoplasma gondii infection in sheep and goats slaughtered for human consumption in Central Ethiopia. BMC Research Notes, 7 (1). doi:

Gebretensay, A., Alemayehu, G., Rekik, M., Alemu, B., Haile, A., Rischkowsky, B. et. al. (2019). Risk factors for reproductive disorders and major infectious causes of abortion in sheep in the highlands of Ethiopia. Small Ruminant Research, 177, 1–9. doi:

Bahiru, A., Assefa, A., Alemu, B., Desta, H., Wieland, B., Sahle, A. (2020). A Case Control Study of Infectious and Non-Infectious Causes of Abortion in sheep and Goats of Abergelle and Ziquala Districts of Amhara Region, Northern Ethiopia. Proceedings of the 12th Annual Regional Conference on Completed Livestock Research Activities. Bahir Dar.

Kaabia, N., Letaief, A. (2009). La fièvre Q en Tunisie. Pathologie Biologie, 57 (5), 439–443. doi:

Angelakis, E., Raoult, D. (2010). Q fever. Veterinary Microbiology, 140 (3-4), 297–309. doi:

de Rooij, M. M. T., Schimmer, B., Versteeg, B., Schneeberger, P., Berends, B. R., Heederik, D., van der Hoek, W., Wouters, I. M. (2012). Risk Factors of Coxiella burnetii (Q Fever) Seropositivity in Veterinary Medicine Students. PLoS ONE, 7 (2), e32108. doi:

Schelling, E., Diguimbaye, C., Daoud, S., Nicolet, J., Boerlin, P., Tanner, M., Zinsstag, J. (2003). Brucellosis and Q-fever seroprevalences of nomadic pastoralists and their livestock in Chad. Preventive Veterinary Medicine, 61 (4), 279–293. doi:

Steinmann, P., Bonfoh, B., Peter, O., Schelling, E., Traore, M., Zinsstag, J. (2005). Seroprevalence of Q-fever in febrile individuals in Mali. Tropical Medicine and International Health, 10 (6), 612–617. doi:

Mazyad, S. A. M., Hafez, A. O. (2007). Q fever (Coxiella burnetii) among man and farm animals in North Sinai, Egypt. Journal of the Egyptian Society of Parasitology, 37 (1), 135–141.

Philip, C. B., Hoogstraal, H., Reiss-Gutfreund, R., Clifford, C. M. (1966). Evidence of rickettsial disease agents in ticks from Ethiopian cattle. Bull World Health Organazation, 35 (2), 127–131.

Endrias, G., Daniel, G. (2014). Seroprevalence of Toxoplasma gondii Infection in Sheep and Goats in Three Districts of Southern Nations, Nationalities and Peoples’ Region of Ethiopia. World Applied Sciences Journal, 31 (11), 1891–1896.

Q Fever OIE Terrestrial Manual 2015 (2015). OIE.

Ullah, Q., Jamil, H., Qureshi, Z. I., Saqib, M., Heinrich, N. (2018). Sero-Epidemiology of Q Fever (Coxiellosis) in Small Ruminants Kept at Government Livestock Farms of Punjab, Pakistan. Pakistan Journal of Zoology, 51 (1). doi:

Awah-Ndukum, J., Mouiche, M. M. M., Bayang, H. N., Ngwa, V. N., Assana, E., Feussom, K. J. M. et. al. (2018). Seroprevalence and Associated Risk Factors of Brucellosis among Indigenous Cattle in the Adamawa and North Regions of Cameroon. Veterinary Medicine International, 2018, 1–10. doi:

Zinsstag, J., Schelling, E., Solera, J., Blasco, J. M., Moriyón, I. (2011). Brucellosis. Oxford Medicine Online. doi:

Radostits, O. M., Gay, C., Blood D. C., Hinchclift, K. W. (2007). Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs and Goats. Elsevier, 966–994.

Gumi, B. (2013). Seroprevalence of Brucellosis and Q-Fever in Southeast Ethiopian Pastoral Livestock. Journal of Veterinary Science & Medical Diagnosis, 02 (01). doi:

Dabassa, G., Tefera, M., Addis, M. (2012). Small Ruminant Brucellosis: Serological Survey in Yabello District, Ethiopia. Asian Journal of Animal Sciences, 7 (1), 14–21. doi:

Sánchez, J., Souriau, A., Buendía, A. J., Arricau-Bouvery, N., Martínez, C. M., Salinas, J. et. al. (2006). Experimental Coxiella burnetii Infection in Pregnant Goats: a Histopathological and Immunohistochemical Study. Journal of Comparative Pathology, 135 (2-3), 108–115. doi:

Arserim, N. B. (2011). Seroprevalance of Coxiellosis in cows, sheep, goats and humans in Diyarbakir region of Turkey. African Journal of Microbiology Research, 5 (15). doi:

Alemnew, E., Yitagesu, E., Goshme, S., Aydefruhim, D. (2020). Retrospective study on kid mortality and associated risk factors of kid survival of newly introduced Boer goat breed in North Shewa Ethiopia. Livestock research for rural development, 32 (7).

Alemnew, E., Yitagesu, E., Addis, H., Areaya, A. (2020). Retrospective Epidemiological Study on Goat Disease in Debre Birhan Agricultural Research Center Ataye Boer Breeding, Evaluation and Distribution Site, North Shoa, Ethiopia. Biomedicine and Nursing, 6 (1), 1–10. doi:

Fekadu, K. (2015). Ethiopian seasonal rainfall variability and prediction using canonical correlation analysis (CCA). Earth Sciences, 4( 3), 112–119.

Bovine brucellosis (2009). OIE Terrestrial Manual, 2, 3–4.

Zegeye, A., Belayineh, N., Shenkutie, A., Lakew, M., Tesfaye, A. (2014). On-Station Retrospective Epidemiologic Study of Small Ruminant Disease in Sirinka Agricultural Research Center, Sheep and Goat Breeding, Evaluation and Distribution Site. Academic Journal of Animal Diseases, 3 (3), 39–44. Available at:

Yizengaw, L., Belayneh, N., Zegeye, A., Tesema, Z., Kefale, A., Kiross, S. et. al. (2020). Retrospective Analysis of Clinical Cases of Caprine and Ovine in Sirinka Agricultural Research Centre, Amhara Region. Proceedings of the 12th Annual Regional Conference on Completed Livestock Research Activities. Bahir Dar.

Hemayatul Islam, Md. (2015). Retrospective Study of Reproductive Diseases of Small Ruminants in Northern Barind Tract in Bangladesh. Animal and Veterinary Sciences, 3 (5), 136. doi:

Sultan, A., Islam, M. R., Yadav, R. K., Akhter, R., Ahmed, J. U. (2015). Prevalence of different reproductive disorders of small ruminants in five upazillas of Mymensingh district. Asian Journal of Medical and Biological Research, 1 (1), 74–79. doi:

Peter, I. D. (2015). A Retrospective Study of Small Ruminant Diseases Identified at the State Veterinary Hospital Maiduguri, Nigeria. Journal of Animal Health and Production, 3 (4), 88–93. doi:

Ahmed, Y. F., Sokkar, S. M., Desouky, H. M., Soror, A. H. (2008). Abortion due to Toxoplasmosis in small ruminants. Global Veterinaria, 2 (6), 337–342. Available at:

Klaasen, M., Roest, H.-J., van der Hoek, W., Goossens, B., Secka, A., Stegeman, A. (2014). Coxiella burnetii Seroprevalence in Small Ruminants in The Gambia. PLoS ONE, 9 (1), e85424. doi:

Tegegne, D., kelifa, A., Abdurahaman, M., Yohannes, M. (2016). Seroepidemiology and associated risk factors of Toxoplasma gondii in sheep and goats in Southwestern Ethiopia. BMC Veterinary Research, 12 (1). doi:

Tilahun, B., Tolossa, Y. H., Tilahun, G., Ashenafi, H., Shimelis, S. (2018). Seroprevalence and Risk Factors of Toxoplasma gondii Infection among Domestic Ruminants in East Hararghe Zone of Oromia Region, Ethiopia. Veterinary Medicine International, 2018, 1–7. doi:

Muma, J. B., Samui, K. L., Siamudaala, V. M., Oloya, J., Matope, G., Omer, M. K. et. al. (2006). Prevalence of antibodies to Brucella spp. and individual risk Factors of Infection in Traditional Cattle, Goats and Sheep Reared in Livestock–Wildlife Interface Areas of Zambia. Tropical Animal Health and Production, 38 (3), 195–206. doi:

Tadesse, G. (2016). Brucellosis Seropositivity in Animals and Humans in Ethiopia: A Meta-analysis. PLOS Neglected Tropical Diseases, 10 (10), e0005006. doi:

Teshale, S., Yibeltal, M., Abaineh, D., Awoke K. (2006). Sero-prevalence of small ruminant brucellosis in selected districts of Afar and Somali pastoral areas of eastern Ethiopia: the impact of husbandry practice. Revue de Medicine Veterinaire, 156, 547–553.

Ashenafi, F., Teshale, S., Agga, G. E., Fikru, R., Laikemariam, Y. (2007). Distribution of brucellosis among small ruminants in the pastoral region of Afar, eastern Ethiopia. Revue Scientifique et Technique de l’OIE, 26 (3), 731–739. doi:

Megersa, B., Biffa, D., Abunna, F., Regassa, A., Godfroid, J., Skjerve, E. (2010). Seroprevalence of brucellosis and its contribution to abortion in cattle, camel, and goat kept under pastoral management in Borana, Ethiopia. Tropical Animal Health and Production, 43 (3), 651–656. doi:

Teklye, B., Kasali, O. (1989). The prevalence of brucellosis in small ruminants in central Ethiopia. Bulletin AHPA, 37, 97–98.

Nakouné, E., Debaere, O., Koumanda-Kotogne, F., Selekon, B., Samory, F., Talarmin, A. (2004). Serological surveillance of brucellosis and Q fever in cattle in the Central African Republic. Acta Tropica, 92 (2), 147–151. doi:

Ullah, Q., El-Adawy, H., Jamil, T., Jamil, H., Qureshi, Z. I., Saqib, M. et. al. (2019). Serological and Molecular Investigation of Coxiella burnetii in Small Ruminants and Ticks in Punjab, Pakistan. International Journal of Environmental Research and Public Health, 16 (21), 4271. doi:

Paul, S., Agger, J. F., Markussen, B., Christoffersen, A.-B., Agerholm, J. S. (2012). Factors associated with Coxiella burnetii antibody positivity in Danish dairy cows. Preventive Veterinary Medicine, 107 (1-2), 57–64. doi:

Rizzo, F., Vitale, N., Ballardini, M., Borromeo, V., Luzzago, C., Chiavacci, L., Mandola, M. L. (2016). Q fever seroprevalence and risk factors in sheep and goats in northwest Italy. Preventive Veterinary Medicine, 130, 10–17. doi:

Kamani, J., Mani, A. U., Egwu, G. O. (2009). Seroprevalence of Toxoplasma gondii infection in domestic sheep and goats in Borno state, Nigeria. Tropical Animal Health and Production, 42 (4), 793–797. doi:

Negash, T., Tilahun, G., Patton, S., Prevot, F., Dorchies, P. (2004). Serological survey of toxoplasmosis in sheep and goats in Nazareth, Ethiopia. Revue de Médecine Véterinaire, 154, 476–477.

Teshale, S., Dumètre, A., Dardé, M. L., Merga, B., Dorchies, P. (2007). Serological survey of caprine toxoplasmosis in Ethiopia: prevalence and risk factors. Parasite, 14 (2), 155–159. doi:

van der Puije, W. N. A., Bosompem, K. M., Canacoo, E. A., Wastling, J. M., Akanmori, B. D. (2000). The prevalence of anti-Toxoplasma gondii antibodies in Ghanaian sheep and goats. Acta Tropica, 76 (1), 21–26. doi:

Bisson, A., Maley, S., Rubaire-Akiiki, C. M., Wastling, J. M. (2000). The seroprevalence of antibodies to Toxoplasma gondii in domestic goats in Uganda. Acta Tropica, 76 (1), 33–38. doi:

Jittapalapong, S., Sangvaranond, A., Pinyopanuwat, N., Chimnoi, W., Khachaeram, W., Koizumi, S., Maruyama, S. (2005). Seroprevalence of Toxoplasma gondii infection in domestic goats in Satun Province, Thailand. Veterinary Parasitology, 127 (1), 17–22. doi:

Swai, E. S., Kaaya, J. E. (2012). A survey of Toxoplasma gondii antibodies by latex agglutination assay in dairy goats in Northern Tanzania. Tropical Animal Health and Production, 45 (1), 211–217. doi:

Barakat, A. M. A., Abd Elaziz, M. M., Fadaly, H. A. (2009). Comparative diagnosis of toxoplasmosis in Egyptian small ruminants by indirect hemagglutination assay and ELISA. Global Veterinaria, 3 (1), 09–14.

Shaapan, R. M., Hassanain, M. A., Khalil, F. A. M. (2010). Modified Agglutination Test for Serologic Survey of Toxoplasma gndii Infection in Goats and Water Buffaloes in Egypt. Research Journal of Parasitology, 5 (1), 13–17. doi:

Opsteegh, M., Teunis, P., Mensink, M., Züchner, L., Titilincu, A., Langelaar, M., van der Giessen, J. (2010). Evaluation of ELISA test characteristics and estimation of Toxoplasma gondii seroprevalence in Dutch sheep using mixture models. Preventive Veterinary Medicine, 96 (3-4), 232–240. doi:

Apparent prevalence of brucellosis, Q-fever and toxoplasmosis in aborted goat’s at North Shoa, Ethiopia

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How to Cite
Alamerew, E. A., Yitagesu, E., Areaya, A., & Aydefruhim, D. (2022). Apparent prevalence of brucellosis, Q-fever and toxoplasmosis in aborted goat’s at North Shoa, Ethiopia. EUREKA: Life Sciences, (5), 28-37.
Environmental Science