Application of bacillus megaterium for subclinical mastitis in cows

Keywords: cows, subclinical mastitis, gastrointestinal microflora, number of somatic cells

Abstract

The problem of treatment of subclinical mastitis often arises after calving, especially in cows of the first lactation. The paper presents the results of the therapeutic effect of Bacillus megaterium in subclinical mastitis in cows.

The aim of the research. To determine the effect of Bacillus megaterium on the microflora of the gastrointestinal tract and colonies of microorganisms isolated from subclinical mastitis in cows.

Materials and methods. The research was conducted in a dairy farm growing Holstein. Cows with subclinical mastitis of the experimental groups were given concentrated feed with the addition of Bacillus megaterium (1 × 109 CFU/g) at a dose of 15–35 g per animal. The control group of cows was given the usual diet for dairy cows. The experiment lasted 30 days.

Results. The use of Bacillus megaterium (1 × 109 CFU/g) at a dose of 35 g per animal had a pronounced effect on the microflora of the gastrointestinal tract of cows. The amount of Lactobacillus sp. was 67 % higher and Bifidobacterium 58 % higher than the control. In addition, the level of opportunistic pathogens on Escherichia coli decreased by 45 %, Clostridium by 27 %, Enterobacteriaceae and Staphylococcus by 75 %, and Candida by 80 % compared to controls. The amount of microflora in milk decreased by 40.2 % and the number of somatic cells by 87.9 %.

Conclusions. The therapeutic efficacy of Bacillus megaterium (1 × 109 CFU/g) at a dose of 35 g per animal for 30 days in subclinical mastitis has been proven. After treatment, the amount of microflora in cow's milk decreased by 40.2 % and the number of somatic cells by 87.9 %.

A positive effect on the microflora of the gastrointestinal tract of cows, where the number of Lactobacillus sp. increased by 67 % and Bifidobacterium by 58 %. The level of opportunistic pathogenic microflora decreased by Escherichia coli – by 45 %, Clostridium – by 27 %, Enterobacteriaceae and Staphylococcus – by 75 %, Candida – by 80 %, compared to the control.

Downloads

Download data is not yet available.

Author Biographies

Yaroslav Tytukh, Sumy National Agrarian University

Department of Obstetrics and Surgery

Yurii Musiienko, Sumy National Agrarian University

Department of Obstetrics and Surgery

Natalia Grebenik, Department of Obstetrics and Surgery

Department of Obstetrics and Surgery

References

Sepúlveda-Varas, P., Proudfoot, K. L., Weary, D. M., von Keyserlingk, M. A. G. (2016). Changes in behaviour of dairy cows with clinical mastitis. Applied Animal Behaviour Science, 175, 8–13. doi: http://doi.org/10.1016/j.applanim.2014.09.022

Wang, Y., Nan, X., Zhao, Y., Jiang, L., Wang, M., Wang, H. et. al. (2021). Rumen microbiome structure and metabolites activity in dairy cows with clinical and subclinical mastitis. Journal of Animal Science and Biotechnology, 12 (1). doi: http://doi.org/10.1186/s40104-020-00543-1

Bardhan, D. (2013). Estimates of economic losses due to clinical mastitis in organized dairy farms. Indian Journal of Dairy Science, 66, 168–172.

Shkromada, O., Palii, A., Palii, A., Skliar, O., Dudchenko, Y., Necherya, T. (2019). Improvement of milk quality for micro-climate formation on cattle farms. Bulletin of Sumy National Agrarian University. The Series: Veterinary Medicine, 4 (47), 43–49. doi: http://doi.org/10.32845/bsnau.vet.2019.4.7

Sinha, M. K., Thombare, N. N., Mondal, B. (2014). Subclinical Mastitis in Dairy Animals: Incidence, Economics, and Predisposing Factors. The Scientific World Journal, 2014, 1–4. doi: http://doi.org/10.1155/2014/523984

Clemente, J. C., Manasson, J., Scher, J. U. (2018). The role of the gut microbiome in systemic inflammatory disease. BMJ, j5145. doi: http://doi.org/10.1136/bmj.j5145

Jost, T., Lacroix, C., Braegger, C., Chassard, C. (2013). Assessment of bacterial diversity in breast milk using culture-dependent and culture-independent approaches. British Journal of Nutrition, 110 (7), 1253–1262. doi: http://doi.org/10.1017/s0007114513000597

Ma, C., Sun, Z., Zeng, B., Huang, S., Zhao, J., Zhang, Y. et. al. (2018). Cow-to-mouse fecal transplantations suggest intestinal microbiome as one cause of mastitis. Microbiome, 6 (1). doi: http://doi.org/10.1186/s40168-018-0578-1

Shively, C. A., Register, T. C., Appt, S. E., Clarkson, T. B., Uberseder, B., Clear, K. Y. J. et. al. (2018). Consumption of Mediterranean versus Western Diet Leads to Distinct Mammary Gland Microbiome Populations. Cell Reports, 25 (1), 47–56.e3. doi: http://doi.org/10.1016/j.celrep.2018.08.078

Bhutto, A. L., Murray, R. D., Woldehiwet, Z. (2012). California mastitis test scores as indicators of subclinical intra-mammary infections at the end of lactation in dairy cows. Research in Veterinary Science, 92 (1), 13–17. doi: http://doi.org/10.1016/j.rvsc.2010.10.006

Prescott, S. C., Breed, R. S. (1910). The determination of the number of body cells in milk by a direct method. American Journal of Public Hygiene, 20, 662–640. Available at: https://pubmed.ncbi.nlm.nih.gov/19599514/

Das, D., Panda, S. K., Jena, B., Sahoo, A. K. (2018). Economic Impact of Subclinical and Clinical Mastitis in Odisha, India. International Journal of Current Microbiology and Applied Sciences, 7 (3), 3651–3654. doi: http://doi.org/10.20546/ijcmas.2018.703.422

Abebe, R., Hatiya, H., Abera, M., Megersa, B., Asmare, K. (2016). Bovine mastitis: prevalence, risk factors and isolation of Staphylococcus aureus in dairy herds at Hawassa milk shed, South Ethiopia. BMC Veterinary Research, 12 (1). doi: http://doi.org/10.1186/s12917-016-0905-3

Romero, J., Benavides, E., Meza, C. (2018). Assessing Financial Impacts of Subclinical Mastitis on Colombian Dairy Farms. Frontiers in Veterinary Science, 5. doi: http://doi.org/10.3389/fvets.2018.00273

Kalińska, A., Jaworski, S., Wierzbicki, M., Gołębiewski, M. (2019). Silver and Copper Nanoparticles – An Alternative in Future Mastitis Treatment and Prevention? International Journal of Molecular Sciences, 20 (7), 1672. doi: http://doi.org/10.3390/ijms20071672


👁 289
⬇ 186
Published
2021-10-29
How to Cite
Tytukh, Y., Musiienko, Y., & Grebenik, N. (2021). Application of bacillus megaterium for subclinical mastitis in cows. Technology Transfer: Innovative Solutions in Medicine, 32-34. https://doi.org/10.21303/2585-6634.2021.002137
Section
Veterinary Science and Veterinary Medicine

Most read articles by the same author(s)