Technology transfer: innovative solutions in medicine

PROTO-HORMONES LEVELS OF ADIPOSE TISSUE, INTERLEUKIN-6 AND INDICATORS OF DETOXIFYING FUNCTION OF THE LIVER IN PATIENTS WITH HYPERTENSION AND OBESITY COMBINED WITH NON-ALCOHOLIC FATTY LIVER DISEASE

Olesia Bochar — Thu, 29 Oct 2020 00:00:00 +0200

The research estimates the state of liver detoxifying function and analyzes the changes in the levels of leptin, adiponectin and interleukin-6 in patients with arterial hypertension in combination with obesity and non-alcoholic fatty liver disease.

Aim. The aim of the study is to evaluate levels of proto-hormones adipose tissue, interleukin-6 and indicators of detoxifying function of the liver in patients with hypertension and obesity combined with non-alcoholic fatty liver disease (NAFLD).

Materials and methods. The study involved patients with arterial hypertension combined with obesity and NAFLD. All patients underwent anthropometric, general clinical, laboratory (blood lipid profile) and instrumental diagnostics (electrocardiography, echocardiography, ultrasonography, ¹³C-metacetin breath test, ELISA (adiponectin, leptin, IL-6). The patients’ height and weight were measured, the body mass index was calculated according to standard formulas.

Results. Patients with arterial hypertension combined with obesity and NAFLD at the stage of steatohepatitis showed an increase in the levels of leptin and IL-6 and a decrease in the level of adiponectin. This group also revealed a moderate decrease in liver detoxifying function, as indicated by the results of ¹³C- MBT due to a 46.7 % decrease in the metabolic rate and a decrease in cumulative doses of CUM40 by 40 % and CUM120 by 46.8 %, respectively.

Conclusions. The elevated levels of leptin and IL-6 and lowered adiponectin levels can be used to determine the degree of activity of non-alcoholic steatohepatitis and predict the course of NAFLD in combination with hypertension and obesity.

An increased level of leptin and IL-6 and a low level of adiponectin in patients with such a comorbid pathology lead to an increase in the left ventricular myocardial mass index and aggravate the course of arterial hypertension.

EVALUATION OF COGNITIVE FUNCTIONS IN NEURODEGENERATION

Maxim Chernenko — Thu, 29 Oct 2020 00:00:00 +0200

Cognitive functions and their disorders in patients with neurodegenerative diseases are an urgent problem of modern neurology. Such functions include memory, attention, praxis, gnosis, language, thinking, control functions. The study of the neuropsychological profile allows a more accurate picture of the involvement in the pathological process of various brain structures and thus facilitate the differential diagnosis. The aim of the research: on the basis of studying of cognitive features of patients with neurodegenerative (demyelinating) pathology to consider possibilities of their psychoneurological adaptation. Materials and methods. The survey data of 104 patients with remitting type of multiple sclerosis, who were treated at the state institution “Institute of Neurology, Psychiatry and Addiction of the National Academy of Medical Sciences of Ukraine” are presented. To study the disorders of verbal memory, the method of A. R. Luria “Memorization of 10 words” was used, which allows to estimate such parameters as the amount of direct memory and the strength of delayed memory (i.e. indicators of short-term and long-term memory). The Schulte Table method was used to study arbitrary attention disorders. This technique allows you to evaluate such characteristics of random attention as volume, functions of concentration distribution and switching of attention.

Results. The data obtained indicate that, depending on the severity of relapses, there are significant differences in the course of mnestic processes. It was found that in severe variants of remissions in women with relapsing-remitting multiple sclerosis (RRMS) more pronounced than in men, there is a lack of ability to work and exhaustion.

Conclusions. It is concluded that the assessment of cognitive deficit out of connection with personal characteristics and emotional and volitional disorders significantly reduces the informativeness and ability to correct cognitive dysfunction. Timely detection of cognitive and emotional-personal changes can not only improve the quality of life of patients with neurodegenerative diseases, but also act as an independent part of the psychoneurological model of adaptation of this category of patients.

THE EFFICACY OF INTRATHECAL DEXAMETHASONE TO PREVENT EARLY COMPLICATIONS OF SPINAL ANAESTHESIA FOR ELECTIVE CAESAREAN SECTION

Nataliya Pyasetska — Thu, 29 Oct 2020 00:00:00 +0200

Spinal anaesthesia is commonly used for elective caesarean section. But it has some disadvantages and complications in intra- and postoperative period.

The aim of this study was to explore the efficacy of intrathecal or intravenous dexamethasone to prevent some early complications of spinal anaesthesia such as arterial hypotonia, nausea, vomiting, bradycardia, shivering etc.

Material and methods: there were examined 154 healthy, not obese women, ASA I–II, 18–36 years old, 36–40 weeks of gestation, undergoing elective caesarean section under spinal anaesthesia. All patients were divided into three equal groups for randomized, prospective, double-blinded, placebo-controlled clinical trial.

The women of each group received intrathecal hyperbaric bupivacaine 0.5 % 10 mg. Group B (n=51) additionally received intrathecal 1 ml of normal saline=placebo; Group BD (n=52) additionally received 4 mg (1 ml) intrathecal dexamethasone, and Group D (n=51) received 8 mg intravenous dexamethasone directly after spinal puncture. The patients were evaluated for blood pressure, heart rate, nausea, vomiting, shivering or other complications during intra- or postoperative period (24 h). The complications that required medicines correction were recorded and cured.

Results: the addition of intrathecal dexamethasone in Group BD vs Group B significantly decreased frequency and manifestations of arterial hypotonia and nausea (Pearson's χ²=0.486 and χ²=0.479, p<0.05) in intra- and postoperative period after the spinal anaesthesia in elective caesarean section. Intrathecal dexamethasone in Group BD vs Group B significantly reduced shivering (Pearson's χ²⁼0.316, p<0.05) in intra- and postoperative period, and significantly didn`t impact on vomiting and bradycardia.

Conclusions: the addition of 4 mg intrathecal dexamethasone as an adjuvant for spinal anaesthesia can significantly decrease frequency and manifestations of arterial hypotonia and nausea, reduce shivering during perioperative period. The addition of 8 mg intravenous dexamethasone has not the same quality.

THE TACTICS OF ANTIVIRAL THERAPY FOR CHRONIC VIRAL HEPATITIS B IN PREGNANT WOMEN

Yuliia Mudra — Thu, 29 Oct 2020 00:00:00 +0200

The aim of the study. Analysis of tactics of antiviral therapy for chronic viral hepatitis b in pregnant women.

Methods. Theoretical analysis of scientific literature; analysis and generalization. Statistics and comparisons. Classification of theoretical material and development of recommendations.

Research results. Today, there are about 2 billion people in the world ill with a chronic infection caused by the hepatitis B virus, 350 million of whom suffer from chronic hepatitis B, and most are asymptomatic carriers of the Australian antigen (HBsAg). Up to 50 % of all new cases of hepatitis B virus infection are due to vertical infection. Despite the lack of increase in viral load during pregnancy, alanine aminotransferase tends to increase in late pregnancy and in the postpartum period. A sharp drop in postpartum corticosteroids may create favourable conditions for hepatitis B virus activation. It is emphasized that the current treatment of hepatitis B virus includes the use of antiviral drugs, where Peg-IFN is absolutely contraindicated in pregnancy, lamivudine and entecavir are classified by the FDA as category C, and tenofovir and telbivudine are classified as category B. During pregnancy, it is recommended to use mainly category B drugs.

Conclusions. The use of antiviral therapy in combination with immunoprophylaxis of new-borns is the optimal strategy for implementation as a universal program, as the success of such an intervention can make a significant contribution to achieving the ultimate goal of global elimination of hepatitis B virus.

FEATURES OF REPRODUCTIVE HEALTH OF WOMEN WITH LOW BIRTH WEIGHT AND OVERWEIGHT AT BIRTH

Nataliia Nestertsova, Larysa Nazarenko — Thu, 29 Oct 2020 00:00:00 +0200

The aim of the study was to clarify obstetric and perinatal risk in women born with low or overweight.

Materials and methods. 343 women with birth weight abnormalities were examined. From them 3 groups are made, according to criterion “weight of the woman at a birth”: I – 107 women with low weight at a birth, II – 126 with excess, III – 110 people with normal weight. The anamnesis, in particular, family and own perinatal, as well as nutritional support, anthropometric data, the structure of pregnancy complications, the rate of growth and development of the fetus, perinatal results were determined. Levels of eicosapentaenoic and docosahexaenoic acids were determined as markers of nutritional support of the organism, as indicators of angiogenesis were determined – concentration of vascular endothelial growth factor and placental growth factor in blood, morpho-functional studies of placenta were performed.

Results. Women born underweight are more likely to have menstrual disorders (every 4), infertility (every 10), miscarriage (every 5) and gynecological diseases. The pathology of gestation in such women and their mothers is similar in spectrum, which is an example of intrauterine programming. The implementation of this program is facilitated by negative factors of nutritional provision: inadequate breastfeeding, economical type of nutrition in favor of restricting protein foods and deviations in the process of angiogenesis.

Conclusions. The importance of perinatal history as a factor that determines the increased risk of menstrual disorders, the frequency of infertility, the pathological course of gestation during pregnancy, the predisposition to placental insufficiency and the birth of a child with low weight.

Women born with low birth weight and overweight are a contingent of increased perinatal risk, which is associated with a complicated own perinatal history on the background of obstetric pathology in their mothers. This fact complements the concept of intrauterine programming.

INFLUENCE OF EXOCRINE PANCREATIC INSUFFICIENCY ON THE COURSE OF TYPE 2 DIABETES MELLITUS IN PATIENTS WITH PRIMARY OSTEOARTHRITIS

Liliya Babinets, Iryna Halabitska — Thu, 29 Oct 2020 00:00:00 +0200

The study of the functional capacity of the pancreas in chronic pancreatitis (CP) in comorbidity with type 2 diabetes mellitus (DM2) and primary osteoarthritis (OA) is also relevant given that patients with this combination are associated with the presence of metabolic syndrome.

The aim of the study was to investigate the state of functional capacity of the pancreas in patients with comorbid chronic pancreatitis and type 2 diabetes mellitus with osteoarthritis.

Material and methods. 117 patients with CP with OA with concomitant DM2 and without it were studied. The main group consisted of 92 outpatients with CP with OA in combination with diabetes in the phase of stable or unstable remission, the comparison group – 25 patients with isolated CP with primary OA, and the control group – 30 healthy individuals. Diagnoses of OA, CP and DM2 were established according to generally accepted criteria and protocols

Results. Analysis of the data showed that on average in the contingent of studied patients with comorbid OA, CP and DM2 excretory insufficiency of pancreas was significantly more severe than in the group of patients with isolated CP with OA.

Conclusions. In the combined course of CP and DM2 with OA, the correlation between fecal α-elastase-1 and glycosylated hemoglobin was statistically significantly stronger than in the group of patients with isolated CP, indicating a mutually aggravating effect of CP and DM2.

OMENTIN METABOLISM AND PATHOPHYSIOLOGICAL RELATIONSHIP BETWEEN ITS LEVEL AND THE SEVERITY OF LESIONS IN THE INTIMA-MEDIA THICKNESS OF THE CAROTID ARTERIES IN TYPE 2 DIABETES MELLITUS IN COMBINATION WITH OBESITY

Roman Kulynych, Olena Soloviuk , Oleksandr Soloviuk — Thu, 29 Oct 2020 00:00:00 +0200

The relationship of visceral obesity and excess body weight (EBW) with the development of cardiovascular diseases, type 2 diabetes mellitus (DM) and effect on quality and duration of life determine the relevance of studying this problem.

The aim: to assess omentin-1 metabolism and pathophysiological relationship between its level and the severity of lesions in the intima-media thickness (IMT) of the carotid arteries in type 2 DM in combination with EBW and obesity.

Materials and methods. We examined 98 people with DM, the first group consisted of 64 people with EBW and obesity, second group consisted of 34 people with normal body weight, control group – 28 healthy individuals. The concentration of omentin-1 was investigated by ELISA by solid-phase enzyme-linked immunosorbent assay in vitro. Ultrasound of the carotid arteries was performed in the lying position of the patient on the device MyLab50X with a linear sensor of 7 MHz with a slight deviation of the patient's head in the opposite direction.

Results. Evaluation of the correlation matrix showed the presence of a positive rather strong negative connection between IMT, especially the right carotid artery and the level of omentin-1 in the blood (Rs = –0.55; p = 0.002). The largest number of individuals with omentin-1 levels from 5–10 ng / ml had significant disorders of the vascular wall (IMT from 1.0 to 1.2 mm).

Conclusion. Obtained data indicate a statistically significant associative relationship between the dynamics of the predictor of cardiovascular risk – IMT with the degree of impaired expression of omentin-1.

WAYS OF IMPROVING THE RESULTS OF SURGICAL TREATMENT OF GASTRIC MALIGNANT TUMORS, COMPLICATED BY GASTROINTESTINAL BLEEDING IN THE CONDITIONS OF EMERGENCY CARE

Pavlo Ivanchov, Oksana Prudnikova, Anton Kurbanov — Thu, 29 Oct 2020 00:00:00 +0200

The aim is to improve the results of surgical treatment of patients with gastric malignant tumors, complicated by gastrointestinal bleeding, by developing and implementing in clinical practice a new treatment tactic.

Materials and methods. The study was conducted on the basis of the Kyiv City Center for Emergency Care of Patients with Gastrointestinal Bleedings and at the Kyiv City Clinical Ambulance Hospital (Ukraine) for the period from 2010 to 2020. A comprehensive examination and analysis of reatment’s results of 140 patients with malignant gastric tumors complicated by acute gastrointestinal bleeding, which amounted to 2.2 % of all reated patients with gastrointestinal bleeding during this period.

Results. Radical operations were performed in 97 (69.3 %) patients, palliative and symptomatic – in 43 (30.7 %). Comparing the frequency of complications in the two periods of treatment of patients, a decrease in the second period, compared with the first period, the frequency of complications from 27.2 % to 11.4 % due to a decrease of 1.8 times (from 68.8 % to 37,5 %) complications after emergencies and related fatalities from 36.4 % to 0 and 2.2 times (from 20.8 % to 9.6 %) the incidence of complications after early delayed operations with a decrease in frequency fatalities from 20.9 % to 18.2 %.

Conclusions. Operations at the height of acute bleeding in patients with gastric cancer are too dangerous due to the high postoperative mortality. The optimal standard is the use of a set of minimally invasive methods of endosurgical hemostasis to stop active bleeding and prevent its recurrence and operate on patients in the early delayed period. Adherence to such tactics is expedient from the point of view of reduction of risk for a life of the patient and possibility of carrying out radical operations.

MOLECULAR MARKERS OF ENDOGENOUS NEUROPROTECTION IN THE BRAIN OF RATS WITH EXPERIMENTAL PARKINSON'S DISEASE AND ON THE BACKGROUND OF USING NEW PHARMACOTHERAPY SCHEMES

Volodymyr Maramukha — Thu, 29 Oct 2020 00:00:00 +0200

Parkinson's disease (PD) is one of the most common neurodegenerative diseases in the elderly.

The aim of the study. To study apoptotic processes and their role in the formation of dopaminergic neurodegeneration and to develop new treatment regimens with a specific neuroprotective effect on the dopaminergic system.

Materials and methods. The study was carried out on 90 Wistar rats at the age of 6 months weighing 220–290 grams. Parkinsonism was induced by the administration of the neurotoxin MPTP (N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) to experimental rats with neuroprotective treatment: I – Intact (passive control); II – animals with experimental Parkinson's disease (PD, active control); III – PD + Amantadine (AM) IV – PD + AM + Cerebrocurin; V – PD + AM + Pramistar; VI – PD + AM + Gliatilin; VII – PD + AM + Noofen; VIII – PD + AM + Pronoran; IX – PD + AM + Melatonin.

Results. The obtained data indicate that neuroprotective therapy of PD with drugs such as melatonin, cerebrocurin, pronoran and gliatilin in combination with amantadine leads to an increase in the expression of the HIF-1α, HIF-3α, HSP70 genes, bcl-2 proteins and decrease c-fos proteins with caspase-3 as markers of apoptosis and can also serve as a molecular marker for the activation of endogenous neuroprotection mechanisms under the conditions of an experimental PD.

Conclusions. The study experimentally demonstrated a new target of neuroprotection in PD conditions – apoptosis of dopamine-producing neurons and substantiated modulators of this process – drugs for combined therapy with amantadine (melatonin, cerebrocurin, pronoran and gliatilin) as promising drugs for the treatment of PD.

CLINICAL AND PHYSIOLOGICAL JUSTIFICATION OF WEANING FROM MECHANICAL VENTILATION IN CHILDREN WITH RESPIRATORY FAILURE

Olha Filyk — Thu, 29 Oct 2020 00:00:00 +0200

Children frequently are admitted to pediatric intensive care units due to acute respiratory failure and up to 64 % of them need to be mechanically ventilated and later weaned.

The aim was to develop strategy of weaning from mechanical ventilation in children with different types of respiratory failure.

Materials and methods. The work was based on results of treatment of 237 patients aged from 1 month to 18 years old, who were divided into control and study group. Patients of study group were randomized into group I (83 patients) and received traditional ICU monitoring and treatment and group II (79 patients), where was used proposed by us methods of monitoring and treatment.

Results. There was supplemented data on the etiology of respiratory failure in children with taking into account function of diaphragm, changes in hemodynamics, nutritional status, electrolyte balance and central nervous system function. It was established new mechanisms of inadequacy of spontaneous breathing during mechanical ventilation and found causes of unsuccessful weaning from mechanical ventilation. In addition, it was developed a weaning strategy with goal-directed therapy for disorders treatment according the type of respiratory failure and children`s age. As a result, important scientific problem was solved and the results of treatment of children with respiratory failure was improved.

Conclusions. It was found that proposed strategy of weaning from mechanical ventilation allowed to reduce the duration of mechanical ventilation and the frequency of complications (reintubations). There were no significant differences in the frequency of tracheostomy among patients of I and II groups.

FORECASTING THE BURDENED COURSE OF SURGERIES AND EARLY POSTOPERATIVE PERIOD IN PATIENTS WITH PULMONARY TUBERCULOSIS ON THE BASIS OF DENSITOMETRIC ANALYSIS OF PREOPERATIVE COMPUTER TOMOGRAPHY OF CHEST ORGANS

Ihor Kalabukha, Ievgen Maietnyi — Thu, 29 Oct 2020 00:00:00 +0200

Topicality – the success of the use of densitotometry in computed tomography of the lungs to assess the condition of the lungs prompted to determine the possibility of predicting the course of the disease in the surgical treatment of patients with pulmonary tuberculosis.

The aim – to determine densitometric criteria for prognosis of burdened intra- and postoperative course in patients operated on for pulmonary tuberculosis.

Methods – the analysis of preoperative densitometry indices in CT of the lungs in the upper and lower pulmonary fields on the side of the operation in 40 patients operated on for chemoresistant pulmonary tuberculosis with a burdened intra- and postoperative period and the effect of densitometric abnormalities on intra-operative period.

Results – in all examined patients the deviation of values of densitometry from conditional norm was revealed. In most patients with high parenchymal density there was obliteration of the pleural cavity and between the lobular fissures, fibrous changes in the root of the lung, with low density in most cases there was bleeding and insufficient tightness of the parenchymal suture, which together aggravates the early postoperative period.

Conclusions – the relationship between deviations in the density of the lung parenchyma before surgery and the possibility of aggravating factors in the process of surgical treatment of patients with pulmonary tuberculosis was discovered; it was determined that predicting the occurrence of certain aggravating factors in the process of surgical treatment of patients with pulmonary tuberculosis can help prevent complications of the operation or significantly reduce their manifestations.

DEVELOPMENT OF A METHOD TO DETERMINE RELATED IMPURITIES IN BROMIDE 1-(β-PHENYLETHYL)-4-AMINO-1,2,4-TRIAZOLE TABLETS

Natalia Derevianko, Dmytro Skoryna — Thu, 29 Oct 2020 00:00:00 +0200

Aim: to develop a modern method to determine related impurities in bromide 1-(β-phenylethyl)-4-amino-1,2,4-triazole tablets by high performance liquid chromatography (HPLC).

Methods: The development of a method to determine related impurities in bromide 1-(β-phenylethyl)-4-amino-1,2,4-triazole tablets involved an already developed method for the determination of 4-amino-1,2,4-triazole impurities in tablets by HPLC. The test solution and the comparison solution were prepared and chromatographed alternately according to the developed methods. At least three chromatograms were obtained for each solution for the purpose of the reliability of the study results.

Results: Scientists of the Scientific-Production Association "Farmatron" jointly with the staff of the Pharmaceutical Chemistry Department of Zaporizhzhia State Medical University under the leadership of Professor Mazur I. A. obtained a new original compound – bromide 1-(β-phenylethyl)-4-amino-1,2,4-triazole (conditional name "Hypertril"), which exhibits antihypertensive, anti-ischemic and antioxidant properties. It is known that the quality of medicines largely depends on the degree of their purity. Therefore, in accordance with the regulatory documentation and quality control methods of medicines, it is mandatory to carry out tests for impurities. According to the results, the content of impurities of 4-amino-1,2,4-triazole in the tested tablets of bromide 1-(β-phenylethyl)-4-amino-1,2,4-triazole is in the range from 0.049 % to 0.195 % which meets the requirements of regulatory documentation. No peaks of unidentified impurities were detected on the chromatograms of the test solution.

Conclusion: Therefore, a modern method to determine the related impurity of 4-amino-1,2,4-triazole in tablets of bromide 1-(β-phenylethyl)-4-amino-1,2,4-triazole has been developed. The methodology is reproducible, accurate and meets the requirements of regulatory documentation.

RELEASING HORMONE AND VITAMIN EFFICIENCY IN THE TREATMENT OF OBSTETRIC AND GYNECOLOGICAL PATHOLOGIES IN BREEDING SOWS

Alex Chekan, Sergey Khilko — Thu, 29 Oct 2020 00:00:00 +0200

Reproduction is a crucial issue at the present stage in the pig husbandry development. Infertility of breeding sows leads to excessive use of feed, increased costs associated with failed inseminations, insufficient breed and early culling of sows.

Aim of the research. Study of infertility types and prevention of postpartum diseases in sows in order to develop methods to control them.

Methods. A two-stage experiment was carried out in 2019–2020 at the LLC "Ryasnyanske" farm in Sumy region, Ukraine. The first stage involved development of a method for prevention and control of infertility in sows. The second stage focused on the method of postpartum pathology prevention.

Results. The conducted research established that surfagon and vitamins manifested the highest efficiency in case of alimentary infertility as they increased fertilization by 20.8 %. In terms of symptomatic infertility, the best results were achieved through a combined use of surfagon and gonadotropic hormone for both primary and multiple fertilization of sows, especially considering extremely low values in the control group - 15 % and 6.5 % of piglets, respectively. Aminazine proved to be the most effective remedy for immune infertility, the obtained results were 17.2 % higher than in the control group. Surfagon at a dose of 5 ml together with estrofan twice a day fully prevented inflammatory postpartum pathologies in sows.

Conclusions. Pathologies of the reproductive organs in sows are widespread at pork-producing farms. Individual use of surfagon was justified for treatment of alimentary, symptomatic and immune infertility of sows and prevention of postpartum pathologies. A combined use of tissue therapy and biologically active drugs proved to be the most effective. The treatment regimens described above reduce infertility and prevent postpartum pathologies in sows.

INTENSITY OF INFECTION AND MEANS OF GIARDIASIS PREVENTION AT THE FARMS OF UKRAINE

Oksana Shkromada, Tetiana Nedzheria — Thu, 29 Oct 2020 00:00:00 +0200

duodenalis is the most widespread among the six generally recognized species of giardiasis affecting the gastrointestinal tract of mammals. Destruction of Giardia intestinalis oocysts in the environment mitigates the risk of infection for animals and humans.

The objective of the study. The research is aimed at establishing the effectiveness of disinfectants against Giardia intestinalis cysts.

Methods. The research involved twelve farms in Ukraine. Fecal samples were taken from the tested calves aged from 1 to 3 weeks. Giardiasis was diagnosed on the basis of laboratory tests of calf feces by the Fulleborn's method. The object of study was represented by cysts of Giardia intestinalis obtained from the feces through combined flotation methods. After exposure for a period of 30 and 60 minutes, the supernatant was drained and the precipitate applied to a glass slide to be stained with Lugol's solution. Cysts were subject to evaluation in terms of their morphological features. Their shape, size, color, nuclei location and axon were determined under a microscope at a magnification of about 10x400. Yodosol, Kontavir, Biocontact and Bioluft were used as a disinfectant.

Results. The study carried out at dairy farms of various forms of ownership revealed that 25–50 % of calves at the holdings and 50–75 % of those at the farms were infected with Giardia intestinalis. Experiments established that Kontavir at a concentration of 1 %, as a disinfectant, produced a cytotoxic effect (compressed the cyst membrane) on Giardia intestinalis for 60 minutes. If the drug concentration increases to 2–3 %, Kontravir destroys the cyst shell releasing its contents within 30-60 minutes.

Conclusions. The prevalence of Giardia intestinalis has been established through studying dairy farms in four regions of Ukraine. As a disinfectant, Kontavir at a concentration of 1 % produces a cytotoxic effect (compresses the cyst membrane), at a concentration of 2–3 %, it completely destroys the cyst membrane.