Immunohistochemical diagnosis of the viability of the strangulation furrow
The aim of the research was to study the possibility of using immunohistochemical markers for the diagnosis of intravital stangulation.
Materials and methods: Fragments of the neck skin from the strnaglulation zone were selected as objects of research. The main study group included 20 deaths due to hanging (10 men and 10 women). For the control group, 10 cases of acute coronary death (5 men and 5 women) were used. Using a complex of IHC markers, the labeling of the epidermis and epithelial structures, the features of cellular immune responses, the manifestation of oxidative steress were studied.
Results: Peculiarities of morphological manifestations of strangulation furrow in mechanical asphyxia were studied. It is established that the key link of its morphogenesis is impaired vascular wall permeability with loss of type IV collagen in the basement membranes of epithelium and skin vessels, migration into tissues of activated CD15+ granulocytes, CD68+ macrophages and CD117+ labrocytes, endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), fibrinogen protein and transforming growth factor β1.
Conclusion: Such changes in the tinctorial properties of skin and subcutaneous adipose tissue can be recorded by immunohistochemical (IHC) and serve as an important diagnostic criterion for the viability of the formation of the strangulation furrow.
Dziak, L. A., Klyhunenko, O. M. (Eds.) (2019). Mekhanichna asfiksiia. Dnipro: LIRA, 188.
Polutova, N. V., CHesnokova, N. P., Ponukalina, E. V., Bizenkova, M. N. (2017). Asfiksiya: Stadii narusheniya vneshnego dykhaniya, mekhanizmy razvitiya. Nauchnoe obozrenie. Meditsinskie nauki, 2, 57–60.
Viter, V. I., Vavilov, A. Yu., Kungurova, V. V., Babushkina, V. A. (2016). Mekhanicheskaya asfiksiya: sudebno-meditsinskaya diagnostika i otsenka. Izhevsk: GBOU VPO “Izhevskaya gosudarstvennaya meditsinskaya akademiya”, 89.
Molin, Y. A., Andreev, A. A., Vorontsov, G. A. (2019). Morphological artifacts in diagnosis of strangulation asphyxia. Russian Journal of Forensic Medicine, 5 (1), 40–41. doi: http://doi.org/10.19048/2411-8729-2019-5-1-40-41
Morales-Ortíz, J., Deal, V., Reyes, F., Maldonado-Martínez, G., Ledesma, N., Staback, F., Washington, A. V. (2018). Platelet-derived TLT-1 is a prognostic indicator in ALI/ARDS and prevents tissue damage in the lungs in a mouse model. Blood, 132 (23), 2495–2505. doi: http://doi.org/10.1182/blood-2018-03-841593
Van Crombruggen, K., Taveirne, S., Holtappels, G., Leclercq, G., Bachert, C. (2018). Innate lymphoid cells in the upper airways: importance of CD117 and IL-1RI expression. European Respiratory Journal, 52 (6), 1800742. doi: http://doi.org/10.1183/13993003.00742-2018
Avtandilov, G. G. (2002). Osnovy kolichestvennoy patologicheskoy anatomii. Moscow: Meditsina, 240.
Lisyanyi, N. I., Lisyanyi, A. A. (2018). Neutrophils and oncogenesis. Klinicheskaya onkologiya, 8 (1 (29), 40–45.
Springer, M. S., Guerrero-Juarez, C. F., Huelsmann, M., Collin, M. A., Danil, K., McGowen, M. R. et. al. (2020). Genomic and anatomical comparisons of skin support independent adaptation to life in water by cetaceans and hippos. doi: http://doi.org/10.1101/2020.11.15.383638
Zhou, B., Liu, S., He, D., Wang, K., Wang, Y., Yang, T. et. al. (2020). Fibrinogen is a promising biomarker for chronic obstructive pulmonary disease: evidence from a meta-analysis. Bioscience Reports, 40 (7). doi: http://doi.org/10.1042/bsr20193542
Hosseini, J. N., Shahabi, S. H. (2019). Gut Microbiota, Dysbiosis and Immune System; A Brief Review. International Journal of Research in Applied and Basic Medical Sciences, 5 (2), 77–81.
Butov, Yu. S., Skripkin, Yu. K., Ivanov, O. L. (Eds.) (2013). Dermatovenerologiya. Natsioalnoe rukovodstvo. Moscow: GEOTAR-Media, 896.
Mideley, O. D. (2000). Kletochnye mekhanizmy barerno-zaschitnykh funktsіy kozhi i ikh narusheniya pri kozhnoy patologi. Vitebsk: Izdatelstvo Vitebskogo gosudarstvennogo meditsinskogo universiteta, 283.
Guzik, T. J., Skiba, D. S., Touyz, R. M., Harrison, D. G. (2017). The role of infiltrating immune cells in dysfunctional adipose tissue. Cardiovascular Research, 113 (9), 1009–1023. doi: http://doi.org/10.1093/cvr/cvx108
Castoldi, A., Naffah de Souza, C., Câmara, N. O. S., Moraes-Vieira, P. M. (2016). The Macrophage Switch in Obesity Development. Frontiers in Immunology, 6. doi: http://doi.org/10.3389/fimmu.2015.00637
Heo, S.-K., Noh, E.-K., Kim, J. Y., Jeong, Y. K., Jo, J.-C., Choi, Y. et. al. (2017). Targeting c-KIT (CD117) by dasatinib and radotinib promotes acute myeloid leukemia cell death. Scientific Reports, 7 (1). doi: http://doi.org/10.1038/s41598-017-15492-5
Radu, A., Bejenaru, C., Ţolea, I., Maranduca, M., Brănişteanu, D., Bejenaru, L. et. al. (2020). Immunohistochemical study of CD117 in various cutaneous melanocytic lesions. Experimental and Therapeutic Medicine, 21 (1). doi: http://doi.org/10.3892/etm.2020.9510
Lowe, J. S., Anderson, P. G. (2015). Support Cells and the Extracellular Matrix. Stevens Lowes Human Histology, 55–70. doi: http://doi.org/10.1016/b978-0-7234-3502-0.00004-8
Forstermann, U., Sessa, W. C. (2011). Nitric oxide synthases: regulation and function. European Heart Journal, 33 (7), 829–837. doi: http://doi.org/10.1093/eurheartj/ehr304
Kotiuzhynska, S. G., Umanskiy, D. A. (2017). Endothelial dysfunction in the pathogenesis of vascular accidents in cardiovascular diseases. Zaporozhye Medical Journal, 19 (4), 525–530. doi: http://doi.org/10.14739/2310-1210.2017.4.105305
Zaslavskaya, E. L., Morozov, A. N., Ionin, V. A., Ma, I., Nifontov, S. Е., Baranova, Е. I. et. al. (2018). The role of transforming growth factor beta-1 and galectin-3 in formation of the left atrium fibrosis in patients with paroxysmal atrial fibrillation and metabolic syndrome. Russian Journal of Cardiology, 2, 60–66. doi: http://doi.org/10.15829/1560-4071-2018-2-60-66
Levkovich, T. V., Pronko, T. P. (2021). Role of the transforming growth factor β1 in the genesis of arterial hypertension and its complications. Journal of the Grodno State Medical University, 19 (1), 16–22. doi: http://doi.org/10.25298/2221-8785-2021-19-1-16-22
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