The investigation of bacterial adhesion of palatine tonsils epithelial cells in patient with infectious mononucleosis

Keywords: Infectious mononucleosis, children, bacterial tonsillitis, Epstein-Barr virus, bacterial adhesion

Abstract

Aim. To evaluate the features of pharynx tonsils mucous membrane colonization by pathogenic and opportunistic microorganisms by using the pharynx palatine tonsils epithelium electron microscopic examination of the patients with infectious mononucleosis and acute streptococcal tonsillitis.

Materials and methods. Two patients – patient P., 12 years old with a confirmed diagnosis of infectious mononucleosis and patient A., 8 years old with confirmed acute streptococcal tonsillitis. The patients were taken a bacteriological examination of the mucus and epithelium scraping from the surface of the pharyngeal tonsils. Tissue samples were examined in the electron microscopy laboratory of the Lviv National University.

Results and discussion. 36 tonsils epithelial tissue micro preparations of patient A. and 41 micro preparations of patient P. were studied. Streptococcus pyogenes, Str. pneumoniae, Str. viridans, Сandida albicans, as well as non-pathogenic bacteria: Diphtheroides sp., Neisseria sp., Corynebacterium spp. were identified as result of the patient P. bacteriological examination of mucus from the surface of the pharyngeal tonsils. Staph. aureus, Str. viridans, Str. pneumoniae were identified during a bacteriological examination of patient A. Eosinophils with a two-segmented nucleus, specific granularity, phagocytosed spherical bacteria in the cytoplasm were detected during the histological examination of the materials taken from the surface of the patient’s tonsils with acute tonsillitis. Research showed that bacteria were accumulated not only in the structure of extracellular detritus. Numerous bacteria accumulations were also found in the cytoplasm of the epithelial cells in the patient with infectious mononucleosis. The cell's shape looked like a bunch of grapes.

Conclusions. The electron microscopic examination showed differences in the coccal flora localization: the extracellular localization of bacteria in the patient with acute bacterial tonsillitis and intraepithelial presence of the bacteria in the patient with tonsillitis during infectious mononucleosis were found.

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Author Biographies

Aleksandr Nadraga, Danylo Halytsky Lviv National Medical University
Department of pediatric infectious diseases
Aleksandr Lutsyk, Danylo Halytsky Lviv National Medical University

Department of Histology, Cytology and Embryology

Khrystyna Klymenko, Danylo Halytsky Lviv National Medical University

Department of pediatric infectious diseases

Olena Khomyn , Danylo Halytsky Lviv National Medical University

Department of pediatric infectious diseases

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Published
2021-05-25
How to Cite
Nadraga, A., Lutsyk, A., Klymenko, K., & Khomyn , O. (2021). The investigation of bacterial adhesion of palatine tonsils epithelial cells in patient with infectious mononucleosis. EUREKA: Health Sciences, (3), 45-52. https://doi.org/10.21303/2504-5679.2021.001835
Section
Medicine and Dentistry