Histotopographic and morphometric characteristics of the oral mucosa postoperative wounds healing, depending on the method of connecting the wound edges

Keywords: mucous membrane, oral surgery, high-frequency electric welding, nylon, adhesive composition, experiment, histology, morphometry

Abstract

Implementation of high-quality tissue connections and hemostasis in oral surgery is an urgent problem of modern dentistry, due to the lack of available universal methods that would help to facilitate the work of oral surgeon and reduce wound healing time.

The aim of the research. To investigate and study the regeneration processes in the oral mucosa after high-frequency electric welding, suturing and medical adhesive composition.

Materials and methods. This study compared the effect of three connection methods and hemostasis: high-frequency electric welding (EKVZ-300M1 “PATONMED®, Ukraine), suture method (nylon 5/0 “PIRUS®”, China), adhesive composition (“Histoacryl®”, Germany). 72 rabbits were involved in experiment. An incision of the soft tissues of the vestibulum of the oral cavity of the maxilla with a length of 1.3–1.5 cm was made. On days 3, 7, 14 and 21, tissue samples were excised, histological and morphometric examination of the material were performed.

Results. By statistical data processing of 72 rabbits was found that the reliability of differences in the levels of morphometric parameters between groups on each day is very high, as evidenced by the calculated values ​​of the U-test. In 38 cases (90.5 %), the significance level was p≤0.01, in the other 4 cases – p≤0.05. The lowest value level of neutrophilic granulocytes was in rabbits of group No.1 (electric welding). In rabbits of group No. 1 GFBC were not observed in the 8 fields of view throughout the experiment.

Conclusions. High-frequency electric welding of soft tissues can be used for effective intraoperative hemostasis and connection of the intraoral wound edges.

Due to the absence of a foreign body (adhesive masses, filaments) and moderate alterative effect, histological examination indicates accelerated activation of regenerative processes in the early stages of observation in rabbits of group No. 1.

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Author Biographies

Oleksiy Pavlenko, Shupyk National Healthcare University of Ukraine

Department of Dentistry

Mikola Boiko, Shupyk National Healthcare University of Ukraine

Department of Dentistry

Irina Savitskaya, Shalimov National Institute of Surgery and Transplantology National Academy of Medical Sciences of Ukraine

Department of Experimental Surgery

Olga Geylenko, Shalimov National Institute of Surgery and Transplantology National Academy of Medical Sciences of Ukraine

Department of Experimental Surgery

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Published
2021-05-25
How to Cite
Pavlenko, O., Boiko, M., Savitskaya, I., & Geylenko, O. (2021). Histotopographic and morphometric characteristics of the oral mucosa postoperative wounds healing, depending on the method of connecting the wound edges. EUREKA: Health Sciences, (3), 69-78. https://doi.org/10.21303/2504-5679.2021.001845
Section
Medicine and Dentistry