Polarization of macrophages of mice under the influence of lectin from Bacillus subtilis IMV B-7724
Macrophages (Mph) are highly plastic cells that are able to change their functional activity (polarization) and perform their functions in different physiological and pathological processes (including cancer). Changes in the functional activity of Mph can occur due to the action of a number of external stimuli (cytokines, colony-stimulating factors, products of microbial synthesis, etc.).
The aim of the research was to study the effect of lectin from B. subtilis IMV B-7724 on the state of macrophage polarization in intact mice of the Balb/c strain.
The cytotoxic effect of lectin from B. subtilis IMV B-7724 on the peritoneal Mph of intact Balb/c mice was evaluated in vitro; indices, characterizing the functional activity of Mph with M1 and M2 phenotypes and the levels of STAT-1 and STAT-6 mRNA expression, were determined.
We have shown that the effect of bacterial lectin on peritoneal Mph is concentration-dependent: ≥0.1 mg/ml is cytotoxic while 0.02 and 0.05 mg/ml is stimulating. At low concentrations of lectin there is observed a significant increase in the ratio of NO production to the arginase activity of Mph (NO/Arg), which is characteristic of Mph with the M1 phenotype. Changes in the expression of STAT transcription factors under the influence of the lectin were similar to the changes, found under the combined action of LPS and IFN-γ on Mph. The detected changes in the functional activity of peritoneal Mph of intact mice under the influence of low concentrations of the lectin may be due to the changes in the expression of transcription factors of the JAK-STAT signaling pathway. Understanding the mechanisms of action of lectin from B. subtilis IMV B-7724 on Mph will open new perspectives for their modulation/polarization
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