Survey of plant growth promoting and antagonistic traits in winter wheat grain endophytic bacteria

  • Alina Pastoshchuk Educational and Scientific Center "Institute of Biology and Medicine"
  • Yuliia Yumyna Educational and Scientific Center "Institute of Biology and Medicine"
  • Pavlyna Zelena Educational and Scientific Center "Institute of Biology and Medicine"
  • Larysa Skivka Educational and Scientific Center "Institute of Biology and Medicine"
Keywords: wheat grain endophytic bacteria, plant growth promoting traits, antagonistic activity, Pseudomonas syringae pv. atrofaciens (McCulloch)

Abstract

The aim of this work was to isolate endophytic bacteria from wheat grains and to evaluate their plant growth promoting traits (PGPT) as well as an inhibitory effect on P. syringae pv. atrofaciens (McCulloch) growth. Endophytic bacteria were isolated by a culture-dependent protocol from the grains of winter wheat variety of Ukrainian selection Podolyanka with high resistance to syringae. Totally 2.7±0.09 CFU/1 g of dry wheat grain were isolated, ten cultivable bacterial isolates were obtained. Spore-forming bacilli predominated in the wheat grain endophytic community. Gram-negative fermenting and non-fermenting rod-shaped bacteria and Gram-positive cocci were also present. Seven out of ten isolates possessed numerous plant growth promoting traits including phosphate solubilization, oligonitrotrophy, and indolic compound producing. Two isolates possessed antagoniscic activity against syringae in vitro along with plant growth promoting features. According to biochemical profiling and mass-spectrophotometric identification, these two isolates were assigned to Paenibacillus and Brevibacillus genera. These endophytic bacteria can be considered as promising objects for agrobiotechnology. However, more research is needed to confirm their biotechnological potential in planta experiments

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Author Biographies

Alina Pastoshchuk, Educational and Scientific Center "Institute of Biology and Medicine"

Postgraduate Student

Department of Microbiology and Immunology

Yuliia Yumyna, Educational and Scientific Center "Institute of Biology and Medicine"

PhD, Associate Professot

Department of Microbiology and Immunology

Pavlyna Zelena, Educational and Scientific Center "Institute of Biology and Medicine"

Associate Researcher

Department of Microbiology and Immunology

Larysa Skivka, Educational and Scientific Center "Institute of Biology and Medicine"

Doctor of Biological Sciences, Professor

Department of Microbiology and Immunology

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Published
2021-07-30
How to Cite
Pastoshchuk, A., Yumyna, Y., Zelena, P., & Skivka, L. (2021). Survey of plant growth promoting and antagonistic traits in winter wheat grain endophytic bacteria. EUREKA: Life Sciences, (4), 66-72. https://doi.org/10.21303/2504-5695.2021.001978
Section
Immunology and Microbiology