Comprehensive study of selection-value lines of zucchini on the level of damage by the yellow mosaic virus (ZYMV) and manifestation of other diseases

  • Serhii Kondratenko Institute of Vegetable and Melon Growing of the National Academy of Agrarian Sciences of Ukraine
  • Oksana Sergienko Institute of Vegetable and Melon Growing of the National Academy of Agrarian Sciences of Ukraine
  • Yuliya Lancaster Tozer Seeds Ltd.
Keywords: zucchini yellow mosaic virus (ZYMV), zucchini line, correlation analysis, field and laboratory resistance


The work is aimed at the selection of linear samples of zucchini (Cucurbita pepo L. var. Giromontia Duch.) with increased complex resistance to abiotic factors of cultivation – zucchini yellow mosaic virus (ZYMV), powdery mildew, bacteriosis and other viral pathogens. Selection studies on choosing virus-resistant zucchini lines from the United Kingdom, Italy, the United States and Spain have identified a number of important correlations levels of resistance to ZYMV and to harmful microorganisms and viruses, belonging to other genera. The assessment of the resistance of the selected zucchini lines to the complex of studied diseases was performed on a 9-point scale of the classifier of the Council for Mutual Economic Assistance (CMEA). In total, during 2017–2019, 20 lines of zucchini were studied in the field. According to the complex resistance to all pathogens (zucchini yellow mosaic virus, green speckled mosaic, white mosaic and cucumber mosaic, bacteriosis and fusarium wilt), four lines have been identified – LZ 17-11, RVL-19, LZ 17-45 and LZ 17-49, which were resistant at the level of points 7 and 9 on the scale of CMEA. The possibility of selecting potentially resistant linear zucchini genotypes for ZYMV based on correlations with the manifestation of other diseases that occurred in the field has been confirmed. The highest level of correlation 0.59<rp<1.0) is observed when comparing the degree of powdery mildew development and symptoms of ZYMV (17 statistically confirmed values of the pairwise correlation coefficient or 80.95 %). A similar indicator for the pair “bacteriosis/ZYMV” is 14 statistically confirmed values of the pairwise correlation coefficient or 66.67 % (–1.0<rp<0.99), and for the pair “field viruses of other origin/ZYMV” – 11 values, which is 52.38 % (–1.0<rp<0.93). Zucchini samples were identified, in which statistically significant correlations were observed for all pairs of comparative traits. Among them, the variety-standard Chaklun and 4 more lines – LZ 17-1, LZ 17-8, LZ 17-50 and LZ 17-44 (–1.0<rp<0.92). The analysis of correlations shows a complex genetic organization of signs of resistance in zucchini lines to the studied pathogens and a high dependence of the manifestation of these signs on the response of the genotype of the line. One of the proofs of this experimental fact is that in all comparative pairs of indicators of the degree of development of certain diseases there are linear genotypes with both positive and negative values of Pearson correlation coefficients (rp). The high level of statistically significant correlations revealed gives grounds for wide use of correlation analysis in selection work for choosing lines potentially resistant to the complex of the most common diseases in vegetable agrocenoses


Download data is not yet available.

Author Biographies

Serhii Kondratenko, Institute of Vegetable and Melon Growing of the National Academy of Agrarian Sciences of Ukraine

Department of Breeding and Seed Production of Vegetable and Melon Crops

Oksana Sergienko, Institute of Vegetable and Melon Growing of the National Academy of Agrarian Sciences of Ukraine

Scientific Secretary

Yuliya Lancaster, Tozer Seeds Ltd.

Plant Breeder


List of plants in the family Cucurbitaceae (2015). Britannica. Available at:

Yarovyi, H. I., Romanov, O. V. (2017). Ovochivnytstvo. Kharkiv: KhNAU, 376. Available at:

Serginko, O. V. (2020). Zabachok-tsukini: features і technology. Ovoshchi i frukty, 10, 20–24. Available at:

Sydorka, V. A. (2015). Performance evaluation source material zucchini on a set of agronomic features. Ovochivnytstvo i bashtannytstvo, 61, 257–261. Available at:

Palamarchuck, I. I. (2017). Annotation the productivity of plants of vegetable marrow is depending on of high quality features in the conditions of forest-steppe right-bank of Ukraine. Silske hospodarstvo ta lisnytstvo, 1 (7), 150–157. Available at:

Vdovenko, S. A., Palamarchuk, I. I. (2019). Osoblyvosti tekhnolohiyi vyroshchuvannia kabachka v umovakh vidkrytoho hruntu. Vinnytsia: VNAU, 195. Available at:

Hoisiuk, L. V. (2015). Naukove obhruntuvannia tekhnolohiyi vyroshchuvannia kabachka v umovakh Lisostepu Zakhidnoho. Visnyk Zhytomyrskoho natsionalnoho ahroekolohichnoho universytetu, 2 (1), 152–156.

Rudnieva, T. O., Shevchenko, T. P., Boiko, A. L. (2008). Vlastyvosti virusu zhovtoi mozaiky tsukini, izolovanoho z roslyn rodyny Cucurbitaceae. Ahroekolohichnyi zhurnal. Spetsialnyi vypusk, 205–207.

Rudnieva, T., Shevchenko, T., Tsvigun, V., Polishchuk, V. (2013). Monitoring of watermelon mosaic virus 2 in agriecosystems of Kyiv and Poltava regions. Microbiology & Biotechnology, 4 (24), 55–62. doi:

Rudnieva, T. O., Shevchenko, O. P., Bysov, A. N., Polishchuk, V. P. (2008). Poshyrennia virusnykh zakhvoriuvan roslyn roslyn Cucurbitaceae na terytoriyi Ukrainy. Ahroekolohichnyi zhurnal, 2, 62–66.

Rudnieva, T. O., Shevchenko, T. P., Bysov, A. S., Boiko, A. L., Polishchuk, V. P. (2010). Virusy roslyny Cucurbitaceae, shcho tsyrkuliuiut v ahrotsenozakh Ukrainy: rozrobka diahnostykumiv na osnovi imunofermentnoho analizu ta yikh zastosuvannia. Kyiv: DIA, 20.

Lefkowitz, E. J., Dempsey, D. M., Hendrickson, R. C., Orton, R. J., Siddell, S. G., Smith, D. B. (2018). Virus taxonomy: the database of the International Committee on Taxonomy of Viruses (ICTV). Nucleic acids research, 46 (D1), D708–D717. doi:

Fauquet, C., Mayo, M. A., Maniloff, J., Desselberger, U., Ball, L. A. (Eds.) (2005). Virus taxonomy. VIIIth Report of the International Committee on Taxonomy of Viruses. Academic Press, 1162. Available at:

Calil, I. P., Fontes, E. P. B. (2016). Plant immunity against viruses: antiviral immune receptors in focus. Annals of Botany, 119 (5), 711–723. doi:

Ling, K.-S., Harris, K. R., Meyer, J. D. F., Levi, A., Guner, N., Wehner, T. C. et. al. (2009). Non-synonymous single nucleotide polymorphisms in the watermelon eIF4E gene are closely associated with resistance to Zucchini yellow mosaic virus. Theoretical and Applied Genetics, 120 (1), 191–200. doi:

Aruah, C., Uguru, M., Benedict, O. (2011). Nutritional Evaluation of Some Nigerian Pumpkins (Cucurbita Spp). Journal of Fruit, Vegetable and Cereal Science and Biotechnology, 5, 64–71. Available at:

Harth, J. E., Ferrari, M. J., Helms, A. M., Tooker, J. F., Stephenson, A. G. (2018). Zucchini Yellow Mosaic Virus Infection Limits Establishment and Severity of Powdery Mildew in Wild Populations of Cucurbita pepo. Frontiers in Plant Science, 9. doi:

Brown, R. N., Bolanos-Herrera, A., Myers, J. R., Jahn, M. M. (2003). Inheritance of resistance to four cucurbit viruses in Cucurbita moschata. Euphytica, 129, 253–258. doi:

Kondratenko, S. I., Sergienko, O. V., Samovol, O. P., Lancaster, Yu. M. (2021). Results of research on artificial infection of foreign origin zucchini with the yellow mosaic virus (ZYMV). Proceedings of the International scientific-practical conference «Actual problems of agro-science in the context of adaptation to global climate change» devoted to the 75th anniversary of the Doctor of Agricultural Sciences, Professor, Academician of National Academy of Sciences and Academy of Agricultural of Sciences of the Republic of Kazakhstan Meiirman Galiolla. Almatyi: TOO «Asyil KItap» (Baspa uyi). Almalybak, 191–193. Available at:

Hull, R. (2009). Mechanical Inoculation of Plant Viruses. Current Protocols in Microbiology, 13 (1). doi:

Metodyka provedennia fitopatolohichnykh doslidzhen za shtuchnoho zarazhennia Roslyn (2016). Zatverdzheno Nakazom Ministerstva ahrarnoi polityky ta prodo-volstva Ukrainy 12 hrudnia 2016 roku No. 540. Available at:

PM 7/125 (1) ELISA tests for viruses (2015). EPPO Bulletin, 45 (3), 445–449. doi:

Kondratenko, S. I., Lancaster, Y. M. (2021). Methodological aspects of assessing the response of courgette plants to artificial contamination of Zucchini yellow mosaic virus (ZYMV). Achievements of ukraine and the eu in ecology, biology, chemistry, geography and agricultural sciences, 115–134. doi:

Gorovaya, T. K., Tihonova, T. E., Sergeev, G. V., Yarovoy, G. I. (2007). Selekciya, tehnologiya vyraschivaniya i semenovodstvo kabachka i patissona. Kharkiv: IOB UAAN, 22.

Lymar, A. O. (2001). Metodyka selektsiynoho protsesu ta provedennia polovykh doslidzhen z bashtannymy kulturamy: metodychni rekomendatsiyi. Kyiv: Ahrarna nauka, 132.

👁 148
⬇ 128
How to Cite
Kondratenko, S., Sergienko, O., & Lancaster, Y. (2021). Comprehensive study of selection-value lines of zucchini on the level of damage by the yellow mosaic virus (ZYMV) and manifestation of other diseases. EUREKA: Life Sciences, (6), 8-16.
Agricultural and Biological Sciences