Comprehensive study of selection-value lines of zucchini on the level of damage by the yellow mosaic virus (ZYMV) and manifestation of other diseases
The work is aimed at the selection of linear samples of zucchini (Cucurbita pepo L. var. Giromontia Duch.) with increased complex resistance to abiotic factors of cultivation – zucchini yellow mosaic virus (ZYMV), powdery mildew, bacteriosis and other viral pathogens. Selection studies on choosing virus-resistant zucchini lines from the United Kingdom, Italy, the United States and Spain have identified a number of important correlations levels of resistance to ZYMV and to harmful microorganisms and viruses, belonging to other genera. The assessment of the resistance of the selected zucchini lines to the complex of studied diseases was performed on a 9-point scale of the classifier of the Council for Mutual Economic Assistance (CMEA). In total, during 2017–2019, 20 lines of zucchini were studied in the field. According to the complex resistance to all pathogens (zucchini yellow mosaic virus, green speckled mosaic, white mosaic and cucumber mosaic, bacteriosis and fusarium wilt), four lines have been identified – LZ 17-11, RVL-19, LZ 17-45 and LZ 17-49, which were resistant at the level of points 7 and 9 on the scale of CMEA. The possibility of selecting potentially resistant linear zucchini genotypes for ZYMV based on correlations with the manifestation of other diseases that occurred in the field has been confirmed. The highest level of correlation 0.59<rp<1.0) is observed when comparing the degree of powdery mildew development and symptoms of ZYMV (17 statistically confirmed values of the pairwise correlation coefficient or 80.95 %). A similar indicator for the pair “bacteriosis/ZYMV” is 14 statistically confirmed values of the pairwise correlation coefficient or 66.67 % (–1.0<rp<0.99), and for the pair “field viruses of other origin/ZYMV” – 11 values, which is 52.38 % (–1.0<rp<0.93). Zucchini samples were identified, in which statistically significant correlations were observed for all pairs of comparative traits. Among them, the variety-standard Chaklun and 4 more lines – LZ 17-1, LZ 17-8, LZ 17-50 and LZ 17-44 (–1.0<rp<0.92). The analysis of correlations shows a complex genetic organization of signs of resistance in zucchini lines to the studied pathogens and a high dependence of the manifestation of these signs on the response of the genotype of the line. One of the proofs of this experimental fact is that in all comparative pairs of indicators of the degree of development of certain diseases there are linear genotypes with both positive and negative values of Pearson correlation coefficients (rp). The high level of statistically significant correlations revealed gives grounds for wide use of correlation analysis in selection work for choosing lines potentially resistant to the complex of the most common diseases in vegetable agrocenoses
List of plants in the family Cucurbitaceae (2015). Britannica. Available at: https://www.britannica.com/topic/list-of-plants-in-the-family-Cucurbitaceae-2038063
Yarovyi, H. I., Romanov, O. V. (2017). Ovochivnytstvo. Kharkiv: KhNAU, 376. Available at: http://dspace.knau.kharkov.ua/jspui/handle/123456789/2081
Serginko, O. V. (2020). Zabachok-tsukini: features і technology. Ovoshchi i frukty, 10, 20–24. Available at: https://www.pro-of.com.ua/kabachok-cukini-osoblivosti-i-texnologiya/
Sydorka, V. A. (2015). Performance evaluation source material zucchini on a set of agronomic features. Ovochivnytstvo i bashtannytstvo, 61, 257–261. Available at: https://vegetables-journal.com/index.php/journal/article/view/194/278
Palamarchuck, I. I. (2017). Annotation the productivity of plants of vegetable marrow is depending on of high quality features in the conditions of forest-steppe right-bank of Ukraine. Silske hospodarstvo ta lisnytstvo, 1 (7), 150–157. Available at: http://socrates.vsau.org/repository/getfile.php/16731.pdf
Vdovenko, S. A., Palamarchuk, I. I. (2019). Osoblyvosti tekhnolohiyi vyroshchuvannia kabachka v umovakh vidkrytoho hruntu. Vinnytsia: VNAU, 195. Available at: http://socrates.vsau.org/repository/getfile.php/23092.pdf
Hoisiuk, L. V. (2015). Naukove obhruntuvannia tekhnolohiyi vyroshchuvannia kabachka v umovakh Lisostepu Zakhidnoho. Visnyk Zhytomyrskoho natsionalnoho ahroekolohichnoho universytetu, 2 (1), 152–156.
Rudnieva, T. O., Shevchenko, T. P., Boiko, A. L. (2008). Vlastyvosti virusu zhovtoi mozaiky tsukini, izolovanoho z roslyn rodyny Cucurbitaceae. Ahroekolohichnyi zhurnal. Spetsialnyi vypusk, 205–207.
Rudnieva, T., Shevchenko, T., Tsvigun, V., Polishchuk, V. (2013). Monitoring of watermelon mosaic virus 2 in agriecosystems of Kyiv and Poltava regions. Microbiology & Biotechnology, 4 (24), 55–62. doi: https://doi.org/10.18524/2307-4663.2013.4(24).48976
Rudnieva, T. O., Shevchenko, O. P., Bysov, A. N., Polishchuk, V. P. (2008). Poshyrennia virusnykh zakhvoriuvan roslyn roslyn Cucurbitaceae na terytoriyi Ukrainy. Ahroekolohichnyi zhurnal, 2, 62–66.
Rudnieva, T. O., Shevchenko, T. P., Bysov, A. S., Boiko, A. L., Polishchuk, V. P. (2010). Virusy roslyny Cucurbitaceae, shcho tsyrkuliuiut v ahrotsenozakh Ukrainy: rozrobka diahnostykumiv na osnovi imunofermentnoho analizu ta yikh zastosuvannia. Kyiv: DIA, 20.
Lefkowitz, E. J., Dempsey, D. M., Hendrickson, R. C., Orton, R. J., Siddell, S. G., Smith, D. B. (2018). Virus taxonomy: the database of the International Committee on Taxonomy of Viruses (ICTV). Nucleic acids research, 46 (D1), D708–D717. doi: https://doi.org/10.1093/nar/gkx932
Fauquet, C., Mayo, M. A., Maniloff, J., Desselberger, U., Ball, L. A. (Eds.) (2005). Virus taxonomy. VIIIth Report of the International Committee on Taxonomy of Viruses. Academic Press, 1162. Available at: https://www.elsevier.com/books/virus-taxonomy/fauquet/978-0-08-057548-3
Calil, I. P., Fontes, E. P. B. (2016). Plant immunity against viruses: antiviral immune receptors in focus. Annals of Botany, 119 (5), 711–723. doi: https://doi.org/10.1093/aob/mcw200
Ling, K.-S., Harris, K. R., Meyer, J. D. F., Levi, A., Guner, N., Wehner, T. C. et. al. (2009). Non-synonymous single nucleotide polymorphisms in the watermelon eIF4E gene are closely associated with resistance to Zucchini yellow mosaic virus. Theoretical and Applied Genetics, 120 (1), 191–200. doi: https://doi.org/10.1007/s00122-009-1169-0
Aruah, C., Uguru, M., Benedict, O. (2011). Nutritional Evaluation of Some Nigerian Pumpkins (Cucurbita Spp). Journal of Fruit, Vegetable and Cereal Science and Biotechnology, 5, 64–71. Available at: https://www.researchgate.net/publication/233529720_Nutritional_Evaluation_of_Some_Nigerian_Pumpkins_Cucurbita_Spp
Harth, J. E., Ferrari, M. J., Helms, A. M., Tooker, J. F., Stephenson, A. G. (2018). Zucchini Yellow Mosaic Virus Infection Limits Establishment and Severity of Powdery Mildew in Wild Populations of Cucurbita pepo. Frontiers in Plant Science, 9. doi: https://doi.org/10.3389/fpls.2018.00792
Brown, R. N., Bolanos-Herrera, A., Myers, J. R., Jahn, M. M. (2003). Inheritance of resistance to four cucurbit viruses in Cucurbita moschata. Euphytica, 129, 253–258. doi: https://doi.org/10.1023/A:1022224327064
Kondratenko, S. I., Sergienko, O. V., Samovol, O. P., Lancaster, Yu. M. (2021). Results of research on artificial infection of foreign origin zucchini with the yellow mosaic virus (ZYMV). Proceedings of the International scientific-practical conference «Actual problems of agro-science in the context of adaptation to global climate change» devoted to the 75th anniversary of the Doctor of Agricultural Sciences, Professor, Academician of National Academy of Sciences and Academy of Agricultural of Sciences of the Republic of Kazakhstan Meiirman Galiolla. Almatyi: TOO «Asyil KItap» (Baspa uyi). Almalybak, 191–193. Available at: https://kazniizr.kz/wp-content/uploads/2021/06/Sbornik-konf.-75-let.-Mejrman-G.T.-08.06.21.pdf
Hull, R. (2009). Mechanical Inoculation of Plant Viruses. Current Protocols in Microbiology, 13 (1). doi: https://doi.org/10.1002/9780471729259.mc16b06s13
Metodyka provedennia fitopatolohichnykh doslidzhen za shtuchnoho zarazhennia Roslyn (2016). Zatverdzheno Nakazom Ministerstva ahrarnoi polityky ta prodo-volstva Ukrainy 12 hrudnia 2016 roku No. 540. Available at: https://sops.gov.ua/uploads/page/5a5f418eb746e.pdf
PM 7/125 (1) ELISA tests for viruses (2015). EPPO Bulletin, 45 (3), 445–449. doi: https://doi.org/10.1111/epp.12259
Kondratenko, S. I., Lancaster, Y. M. (2021). Methodological aspects of assessing the response of courgette plants to artificial contamination of Zucchini yellow mosaic virus (ZYMV). Achievements of ukraine and the eu in ecology, biology, chemistry, geography and agricultural sciences, 115–134. doi: https://doi.org/10.30525/978-9934-26-086-5-20
Gorovaya, T. K., Tihonova, T. E., Sergeev, G. V., Yarovoy, G. I. (2007). Selekciya, tehnologiya vyraschivaniya i semenovodstvo kabachka i patissona. Kharkiv: IOB UAAN, 22.
Lymar, A. O. (2001). Metodyka selektsiynoho protsesu ta provedennia polovykh doslidzhen z bashtannymy kulturamy: metodychni rekomendatsiyi. Kyiv: Ahrarna nauka, 132.
Copyright (c) 2021 Serhii Kondratenko, Oksana Sergienko, Yuliya Lancaster
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.
Authors, who are published in this journal, agree to the following conditions:
1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the journal under the terms of a Creative Commons CC BY, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this journal.
2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form in which it has been published by the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.