Evaluation of cytotoxic activity of live toxoplasma gondii tachyzoites and toxoplasma antigen on MCF-7 human breast cancer cell line

Keywords: novel chemotherapeutic agents, Toxoplasma tachyzoites, Toxoplasma antigen, MCF-7 cell line

Abstract

The aim of this study was to investigate the cytotoxic potency of live Toxoplasma gondii tachyzoites as well as Toxoplasma antigen on MCF-7 human breast cancer cell line. Cancer cell lines are considered an essential preliminary step towards in-vitro investigation of the potential antineoplastic impact of novel chemotherapeutic agents. Pathogens, including viruses, bacteria, and parasites are noticeably under investigation, considering their potential antineoplastic activity. Some have attained a steady position in the clinical field as hepatitis B virus, human papilloma virus and BCG immunization. Toxoplasma gondii is an apicomplexan parasite with promising antineoplastic activity. In this study, live Toxoplasma tachyzoites provoked a direct cytotoxic effect on MCF-7 in a dose dependent manner, while Toxoplasma antigen didn’t induce such impact.

Skipping the direct cytotoxic effect of Toxoplasma antigen doesn’t totally divert the possible antineoplastic activity of Toxoplasma antigen. Potential alternative immune mediated mechanisms could be an alternative. Further in-vivo studies in different cancer models are mandatory to investigate the underlying mechanisms of antineoplastic activity of Toxoplasma gondii

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Author Biographies

Maha M. Eissa, Alexandria University

Faculty of Medicine

Department of Medical Parasitology

Maha R. Gaafar, Alexandria University

Faculty of Medicine

Department of Medical Parasitology

Layla K. Younis, Alexandria University

Faculty of Medicine

Department of Pathology

Cherine A. Ismail, Alexandria University

Faculty of Medicine

Department of Clinical Pharmacology

Nahla El Skhawy, Alexandria University

Faculty of Medicine

Department of Medical Parasitology

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Published
2022-03-31
How to Cite
Eissa, M. M., Gaafar, M. R., Younis, L. K., Ismail, C. A., & El Skhawy, N. (2022). Evaluation of cytotoxic activity of live toxoplasma gondii tachyzoites and toxoplasma antigen on MCF-7 human breast cancer cell line. EUREKA: Life Sciences, (2), 45-50. https://doi.org/10.21303/2504-5695.2022.002409
Section
Immunology and Microbiology