INDICATORS OF THE ELEMENT RANGE OF RAT’S BLOOD IN TERM ARTIFICIAL HYPOBYOSIS

  • Anna Umanska National University of Life and Environmental Sciences of Ukraine, Ukraine
  • Dmytro Melnichuk Public Organization "The Council of Heroes of Ukraine in the agroindustrial complex", Ukraine
  • Lilia Kalachnyuk National University of Life and Environmental Sciences of Ukraine, Ukraine
Keywords: hypobiosis, hypercapnia, hypoxia, blood, macroelements, microelements

Abstract

The promising method of anesthesia and anesthesia is the state of artificial hypobiosis. Hypercapnia is a prerequisite for creating an artificial carbon dioxide hypobiosis, along with hypoxia and hypothermia. Adhering to the conditions under which the state of artificial carbon dioxide hypobiosis is created, animals reduce the level and intensity of metabolic processes both in the body as a whole and in separate organs. In order to investigate the safety of the hypobiological state and evaluate its applicability in clinical practice, it is necessary to investigate the functional characteristics of the blood. One of the most important indicators is the change of elemental composition. After all, the set of micro-and macro-elements is involved in the enzymatic reactions of the organism, its metabolic processes.

Therefore, the aim of the study was to investigate the elemental changes in the blood of rats for artificial hypobiosis. In experiments, white non-breeding male rats weighing 180–200 g were used, which were maintained under standard vivarium conditions. Animals were divided into 2 groups: control and experimental (the state of artificial hypobiosis). Each group had 7 animals. Measurement of the content of macro- and micronutrients was carried out using a mass spectrometric ionization method in an inductively coupled plasma on an IRIS Interband II XSP device manufactured by Thermo Scientific, USA. The results of the study were processed and presented using the methods of statistical analysis, namely: the method of descriptive statistics; Shapiro-Wielka's criterion for choosing a statistical criterion for comparing groups; Student's criterion; Mann-Whitney's criterion. Results of evaluation of dynamics in the experimental (hypobiotic) group using the criterion of sign-marks of Wilcoxon. As a result of the studies, increased levels of Sodium, Ferrum, Calcium were observed, while the content of Nickel and Barium reduced. The results of the analysis of the dynamics of blood parameters in the experimental group by the methods of descriptive statistics showed significant changes in such elements as: Potassium, Sodium, Ferrum, Calcium, Magnesium. The heterogeneity of the results of the experimental and control groups according to the Mann-Whitney criterion was demonstrated in the following elements: Potassium, Sodium, Ferrum, Calcium, Chromium, Strontium.

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Author Biographies

Anna Umanska, National University of Life and Environmental Sciences of Ukraine

Academician M. F. Guliy Department of Biochemistry

Dmytro Melnichuk, Public Organization "The Council of Heroes of Ukraine in the agroindustrial complex"

Head of Public Organization

Lilia Kalachnyuk, National University of Life and Environmental Sciences of Ukraine

Academician M. F. Guliy Department of Biochemistry

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Published
2018-06-02
How to Cite
Umanska, A., Melnichuk, D., & Kalachnyuk, L. (2018). INDICATORS OF THE ELEMENT RANGE OF RAT’S BLOOD IN TERM ARTIFICIAL HYPOBYOSIS. EUREKA: Life Sciences, (3), 3-12. https://doi.org/10.21303/2504-5695.2018.00657
Section
Agricultural and Biological Sciences