• Tetiana Lakhtadyr O. O. Bogomolets National medical university
Keywords: skin burn injury, infusion solutions, Lactoproteinum with sorbitol, HAES-LX-5 %, structural changes, renal cortex


Aim of research is the establishment of structural features of the course of the adaptive-compensatory and regenerative processes in the cortical substance of the rat kidney in late terms after experimental burn skin injury and the use of HAES-LX-5 % is relevant for theoretical and practical medicine.

Materials and methods.The article presents and analyzes the results of an experimental study on the structural changes of the cortical substance of the kidneys in rats in late terms after burn injury of the kidney with the condition of administration of intravenous infusion of 0.9 % NaCl solution and complex colloid-hyperosmolar solutions of lactoprotein with sorbitol and HAES-LX-5 %. Comparative analysis showed that functionally different cells of the nephrons under the infusion of detoxification solutions in the conditions of development of burn disease die by necrosis, apoptosis and anoikis. Also in epithelial cells of nephron tubules there weremitophagy and mitoptosis.

Result. Mitoptosis in epithelial cells of rat nephron tubules with experimental burn skin injury was carried out in two ways, which are related to I – destruction of the outer mitochondrial membrane; II – preservation of the outer mitochondrial membrane and attachment of autophagic (mitophagic) mechanisms to release the cell from degraded mitochondrial material. In the first case, the mitochondria first condensed, after which its matrix swelled and the fragmentation of the cross occured due to the destruction of the cross compounds. Finally, the outer mitochondrial membrane broke and the remnants of the crystals (in the form of vesicles) went into the cytoplasm. In the second case, the mitochondria condensed, vesicular fragmentation of the sac occured, but the outer mitochondrial membrane retained its integrity and the mitochondria were absorbed by the autophagosome. Next, autophagosomes merged with lysosomes and formed autophagolysosomes, which, subject to effective content processing, were transformed into vacuoles. The latter were emptied by exocytosis and ensured the cell was free from degraded material.

Conclusions. Only a lactoprotein with sorbitol has a membrane-plastic effect aimed at strengthening the mitochondrial membrane, in part of the mitochondria of epithelial cells of nephron tubules is ultrastructurally increased by the electron density and thickness of all components of the mitochondrial membrane. The maximal membrane effect of the action of lactoprotein with sorbitol on the mitochondria manifests itself fourteen days after the experimental burn skin injury and gradually (twenty-one and thirty days later) disappears, which coincides with the improvement of the overall clinical condition. There is every reason to believe that increased structuralization of mitochondria is a safeguard for the spread of mitoptosis and mitophagy, the excessive nature of which can lead to cell death.


Download data is not yet available.

Author Biography

Tetiana Lakhtadyr, O. O. Bogomolets National medical university

PhD, Associate professor

Department of human anatomy


Kruer, R. M., Ensor, C. R. (2012). Colloids in the intensive care unit. American Journal of Health-System Pharmacy, 69 (19), 1635–1642. doi:

Davidson, I. J. (2006). Renal impact of fluid management with colloids: a comparative review. European Journal of Anaesthesiology, 23 (9), 721–738. doi:

Bunn, F., Alderson, Р., Hawkins, V. (2012). Colloid solutions for fluid resuscitation. Cochrane Database of Systematic Reviews, 7, 30–34. doi:

Mohanan, M., Rajan, S., Kesavan, R., Mohamed, Z. U., Ramaiyar, S. K., Kumar, L. (2019). Evaluation of renal function with administration of 6 % hydroxyethyl starch and 4 % gelatin in major abdominal surgeries: a pilot study. Anesthesia: Essays and Researches, 13 (2), 219. doi:

Keck, M., Herndon, D. H., Kamolz, L. P., Frey, M., Jeschke, M. G. (2009). Pathophysiology of burns. Wiener Medizinische Wochenschrift, 159 (13-14), 327–336. doi:

Lachtadyr, T. V. (2019). Structural changes of the rat kidney cortical substance in the long-term period after burn injury of the skin under conditions of HAES-LX_5 % infusion. Emergency Medicine, 5 (100), 96–100.

Lachtadyr, T. V. (2017). Structural changes of rats renal cortex in late period of skin burn injury under the conditions of the infusion by lactoprotein with sorbitol. Biomedical and Biosocial Anthropology, 28, 81–87. Available at:

Groeneveld, A. B., Navickis, R. J., Wilkes, М. М. (2011). Update on the comparative safety of colloids: a systematiс review of clinical studies. Annals of Surgery, 253 (3), 470–483. doi:

Jangamreddy, J. R., Los, M. J. (2012). Mitoptosis, a novel mitochondrial mechanism leading predominantly to activation of autophagy. Hepatitis Monthly, 12 (8), 6159–6163. doi:

Lyamzaev, K. G., Nepryakhina, O. K., Saprunova, V. B., Bakeeva, L. E., Pletjushkina, O. Y., Chernyak, B. V., Skulachev, V. P. (2008). Novel mechanism of elimination of malfunctioning mitochondria (mitoptosis): Formation of mitoptotic bodies and extrusion of mitochondrial material from the cell. Biochimica et Biophysica Acta (BBA) – Bioenergetics, 1777 (7-8), 817–825. doi:

Kovalchuk, O., Cherkasov, E., Dzevulska. I., Raminsky, R., Korsak, A., Sokurenko, L. (2017). Dynamics of morphological changes of rats adenohypophysis in burn disease. Georgian Medical News, 270, 104–108.

Cherkasov, E. V., Gunas, I. V., Chereshnyuk, I. L., Lysenko, D. A. (2012). Features of thymus cells cycle in rats after burn lesion of a skin. Ukrainian morphologycal almanac, 2 (3), 109–113. Available at:

Gunas, I. V., Guminskiy, Y. I., Ocheretn, N. P., Lysenko, D. A., Kovalchuk, О. І., Dzevulska, І. V., Cherkasov, E. V. (2018). Indicators cell cycle and dna fragmentation of spleen cells in early terms after thermal burns of skin at the background of introduction 0.9 % NaCl solution. World of Medicine and Biology, 14 (63), 116–120. doi:

Haagsma, J. A., Graetz, N., Bolliger, I., Naghavi, M., Higashi, H., Mullany, E. C. et. al. (2015). The global burden of injury: incidence, mortality, disability-adjusted life years and time trends from the Global Burden of Disease study 2013. Injury Prevention, 22 (1), 3–18. doi:

Gavryluk, A. O., Galunko, G. M., Chereshniuk, I. L., Tikholaz, V. O., Cherkasov, E. V., Dzevulska, І. V., Kovalchuk, О. І. (2018). Indicators cell cycle and dna fragmentation in cells of small intestine mucosa 14, 21 and 30 days after skin burns on the background of pre-liminary infusion of solution lactoprotein with sorbitol or haes-lx 5 %. World of Medicine and Biology, 14 (63), 104–108. doi:

Hartog, C. S., Kohl, М., Reinhart, K. (2011). A systematic review of third-generation hydroxyethyl starch (HES 130/0.4) in resuscitation: safety not adequated addressed. Anesthesia & Analgesia, 112 (3), 635–645. doi:

Kansir, A. S., Johansen, J. K., Pedersen, Е. В. (2015). The effect of 6 % hydroxyethyl starch 130/0.4 on renal function, arteriol blood pressure, and vasoactive hormones during radical prostatectomy: a randomized controlled trial. Anesth Analg, 120 (3), 608–618. doi:

Dzevulska, І. V., Kovalchuk, О. І., Cherkasov, E. V., Majewskyi, О. Y., Shevchuk, Y. G., Pastukhova, V. A., Kyselova, T. M. (2018). Influence of lactoproteinum solution with sorbitol on dna content of cells of endocrine glands on the background of skin burn in rats. World of Medicine and Biology, 14 (64), 33–39. doi:

👁 192
⬇ 148
How to Cite
Medicine and Dentistry