THE GC-MS DETERMINATION OF CHEMICAL CONSTITUENTS FROM HARPAGOPHYTUM PROCUMBENS DC ROOTS
The present work is devoted to the study of the component composition of the volatile fraction of the heptanoic extract from Harpagophytum procumbens roots (“Starwest Botanicals”, USA).
For this purpose the use of gas chromatography-mass spectrometry (GC-MS) was proposed. The GC-MS analysis was carried out on an Agilent 6890N/5973 inert gas chromatograph/ mass selective detector (Agilent Technologies, USA).
The quantitative content of the components was calculated by the method of the internal standard. The components with the probability of coincidence for mass spectra of more than 90% were taken into account.
As a result of the study more than 31 substances have been identified. Among the main groups of compounds the presence of aromatic hydrocarbons, esters and fatty acids, as well as alkanes and alkenes should be mentioned. Hexadecanoic acid (29.92 mg/kg), Octadec-9-enoic acid (21.39 mg/kg), 8-[4-[N-Aziridyl]butyl]amino-2,6-dimethyl-2,6-octadiene (6.46 mg/kg), Pentacosane (5.85 mg/kg), Hexadecanoic acid, trimethylsilyl ester (4.53 mg/kg) prevail in terms of quantity.Based on the results obtained the conclusion can be made that Harpagophytum procumbens DC is a promising herbal source for obtaining active ingredients with the anti-inflammatory, regenerative, analgesic, immunostimulating properties
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