THE GC-MS DETERMINATION OF CHEMICAL CONSTITUENTS FROM HARPAGOPHYTUM PROCUMBENS DC ROOTS

  • Anna Kriukova National Universiry of Pharmacy 53, Pushkinska str. Kharkiv, 61002, Ukraine
  • Inna Vladimirova National University of Pharmacy 53, Pushkinska str. Kharkiv, 61002, Ukraine
Keywords: Harpagophytum procumbens DC, chemical constituents, chemical structure, gas chromatography combined with mass spectrometry

Abstract

The present work is devoted to the study of the component composition of the volatile fraction of the heptanoic extract from Harpagophytum procumbens roots (“Starwest Botanicals”, USA).

For this purpose the use of gas chromatography-mass spectrometry (GC-MS) was proposed. The GC-MS analysis was carried out on an Agilent 6890N/5973 inert gas chromatograph/ mass selective detector (Agilent Technologies, USA).

The quantitative content of the components was calculated by the method of the internal standard. The components with the probability of coincidence for mass spectra of more than 90% were taken into account.

As a result of the study more than 31 substances have been identified. Among the main groups of compounds the presence of aromatic hydrocarbons, esters and fatty acids, as well as alkanes and alkenes should be mentioned. Hexadecanoic acid (29.92 mg/kg), Octadec-9-enoic acid (21.39 mg/kg), 8-[4-[N-Aziridyl]butyl]amino-2,6-dimethyl-2,6-octadiene (6.46 mg/kg), Pentacosane (5.85 mg/kg), Hexadecanoic acid, trimethylsilyl ester (4.53 mg/kg) prevail in terms of quantity.

Based on the results obtained the conclusion can be made that Harpagophytum procumbens DC is a promising herbal source for obtaining active ingredients with the anti-inflammatory, regenerative, analgesic, immunostimulating properties

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Author Biographies

Anna Kriukova, National Universiry of Pharmacy 53, Pushkinska str. Kharkiv, 61002, Ukraine
Department of Pharmacognosy
Inna Vladimirova, National University of Pharmacy 53, Pushkinska str. Kharkiv, 61002, Ukraine
Department of Pharmacognosy

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Published
2017-10-26
How to Cite
Kriukova, A., & Vladimirova, I. (2017). THE GC-MS DETERMINATION OF CHEMICAL CONSTITUENTS FROM HARPAGOPHYTUM PROCUMBENS DC ROOTS. Technology Transfer: Innovative Solutions in Medicine, 52-54. https://doi.org/10.21303/2585-663.2017.00449
Section
Pharmacology, Toxicology and Pharmaceutical Science