STUDY OF AMINO ACID COMPOSITION IN A THALICTRUM FOETIDUM L. HERB

  • Elena Savelieva Kharkiv National Medical University 4 Nauky ave., Kharkiv, Ukraine, 61022
  • Tatyana Tishakova Kharkiv National Medical University 4 Nauky ave., Kharkiv, Ukraine, 61022
Keywords: Thalictrum foetidum L., herb, biologically active substances, amino acids, high performance liquid chromatography

Abstract

Submitted article is concerned with the study of amino acid composition in a Thalictrum foetidum L. herb. Investigations have been performed by the high performance liquid chromatography method based on the extraction of free amino acids from the herbal raw material and on the acid hydrolysis of the herbal medicinal products followed by analysis of hydrolizates using the HPLC with precolumn derivatization with 9-fluorenylmethoxycarbonyl chloride  (FMOC) and o-phthaldialdehyde (OPA) attended by the detection with fluorescence detector.

Amino acid identification has been performed by the comparison of retention times with the mixture of standard amino acids (Agilent 5061-3334). The content of bound amino acids has been determined by the subtraction of free amino acids composition from their total composition.

As a result of study 15 essential and non-essential amino acids have been found. It is important to note that L-Serine, L-Histidine, Glycine and L-Phenylalanine are not in a free state in Thalictrum foetidum L. herb. The quantity of  L-Glutamic and L-Aspartic acids is 17.47 and 13.41 µg/mg, respectively (common amino acids). The content of abovementioned acids in a bound state is 15.66 and 9.84 µg/mg, respectively. As for content of other amino acids which were found in Thalictrum foetidum L. herb, it has to be said about their practically the same composition which is in the range from 4 to 5 µg/mg. Content of L-Histidine (1.80 µg/mg in general and bound state) and L-Tyrosine (1.76 µg/mg in general state and 0.08 µg/mg in bound state) is minimum in this herb.

It has been established that amino acids are in a free and bound state in the investigated herbal raw material. Content of bound amino acids is higher than general content. Among the amino acids, which are in the overwhelming majority in the studied herb,  it stands to mention L-Glutamic and L-Aspartic. These amino acids play an important role in supporting the physiological processes of the body, especially nervous system.

In consideration of experimental data to be noticed is that Thalictrum foetidum L. is a promising plant for creation of phytotherapeutic medications with neurally mediated action.

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Author Biographies

Elena Savelieva, Kharkiv National Medical University 4 Nauky ave., Kharkiv, Ukraine, 61022
Department of Medical and bioorganic chemistry
Tatyana Tishakova, Kharkiv National Medical University 4 Nauky ave., Kharkiv, Ukraine, 61022

Department of Pharmacognosy

References

Jámbor A., Molnár-Perl I. Amino acid analysis by high-performance liquid chromatography after derivatization with 9-fluorenylmethyloxycarbonyl chloride. Literature overview and further study. Journal of Chromatography A, 1216 (2009) 3064–3077.

Jámbor A., Molnár-Perl I. Quantitation of amino acids in plasma by high performance liquid chromatography: Simultaneous deproteinization and derivatization with 9-fluorenylmethyloxycarbonyl chloride. Journal of Chromatography A, 1216 (2009) 6218–6223.

John W. Henderson, Robert D. Ricker, Brian A. Bidlingmeyer, and Cliff Woodward Rapid, Accurate, Sensitive, and Reproducible HPLC Analysis of Amino Acids Amino Acid Analysis Using Zorbax Eclipse-AAA Columns and the Agilent 1100 HPLC // Agilent Technical Note 1999;5980–1193E.

Гонтова Т. М. Амінокислотний склад густих екстрактів з трави та коренів живокосту шорсткого / Т. М. Гонтова // Актуальні питання фармацевтичної і медичної науки та практики. – 2013. – Т. 12, № 2. –

С. 4–5.

Pollegioni L, Servi S, eds. (2012). Unnatural Amino Acids: Methods and Protocols. Methods in Molecular Biology – Volume 794. Humana Press. p. v.

Michal G, Schomburg D, eds. (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd ed.). Oxford: Wiley-Blackwell. p. 5.

Wolosker H, Dumin E, Balan L, Foltyn VN (July 2008). "D-amino acids in the brain: D-serine in neurotransmission and neurodegeneration". The FEBS Journal. 275 (14): 3514–26.

Brosnan JT (April 2000). "Glutamate, at the interface between amino acid and carbohydrate metabolism". The Journal of Nutrition. 130 (4S Suppl): 988S–90S.

Leuchtenberger W, Huthmacher K, Drauz K (November 2005). "Biotechnological production of amino acids and derivatives: current status and prospects". Applied Microbiology and Biotechnology. 69 (1): 1–8.

Jones RC, Buchanan BB, Gruissem W (2000). Biochemistry & molecular biology of plants. Rockville, Md: American Society of Plant Physiologists. pp. 371–2.


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Published
2017-10-26
How to Cite
Savelieva, E., & Tishakova, T. (2017). STUDY OF AMINO ACID COMPOSITION IN A THALICTRUM FOETIDUM L. HERB. Technology Transfer: Innovative Solutions in Medicine, 58-60. https://doi.org/10.21303/2585-663.2017.00454
Section
Pharmacology, Toxicology and Pharmaceutical Science