PECULIARITIES OF HISTOMORPHOLOGICAL LIVER CHANGES ASSOCIATED WITH COMBINED EFFECT OF ACTIVE SUBSTANCES WITH ONE-PURPOSE ACTION
Abstract
Some studies have proven possible potentiating toxic effect of combined pesticides. The potentiating effect may be related to the impact of a certain substance on enzymatic activity of another one. Due to combined preparations popularity, the importance of evaluating probability of summation and synergetic or antagonizing action of the one-purpose substances becomes particularly important.
Aim of the research is to study peculiarities of histomorphological liver changes associated with combined effect of active substances with one-purpose action.
Methods. The authors have used toxicological, histochemical and statistical study methods. 45 rats have been used for the study. The liver specimens for histochemical analysis were selected immediately after dissection of the rats, from which the 5 micrometer sections were prepared, using freezing microtome. After leaving the sections in cold acetone, the histochemical reaction on determining the γ-glutamyl transpeptidase, a transformed hepatocyte marker, which forms nodules while proliferates, was conducted.
Results. The authors have established that the above-mentioned characteristics in animals which were administered the drug (benthiavalicarb-isopropyl+folpet) do not differ from negative control group. General specific area of nodules per сm2 and specific quantity of nodules per сm2 haven’t reliably changed, the control group exceeding these data by 18 % and 12 %, respectively.
Discussion. The study of the benthiavalicarb-isopropyl and folpet mixture effect on animals has not revealed liver tissue proliferation of the carcinogen-transformed cells and appearance of hyperplastic nodes, which express the γ-glutamyl transpeptidase, a histochemical marker of pre-tumor changes.
So, the fungicide (benthiavalicarb-isopropyl+folpet) has not induced multiplication or size increase of glutamyl transpeptidase nodules, which evidences about loss of the modifying effect of folpet on the benthiavalicarb-isopropyl carcinogenicity in this preparation formula.
The obtained results may be used when deciding on administration of combined fungicides with active substances of one-purpose action.
Downloads
References
Stavnichenko, P. V., Antonenko, A. M., Bardov, V. G. (2017). Forecasting of development of acute poisonings in agricultural workers while using combined formulations based on difenoconazole. Medicni Perspektivi (Medical Perspectives), 22 (3), 116–121. doi: https://doi.org/10.26641/2307-0404.2017.3.111938
Leadbeater, A., Gisi, U. (2009). The Challenges of Chemical Control of Plant Diseases. Recent Developments in Management of Plant Diseases, 3–17. doi: https://doi.org/10.1007/978-1-4020-8804-9_1
Amr, S., Dawson, R., Saleh, D. A., Magder, L. S., St. George, D. M., El-Daly, M. et. al. (2014). Pesticides, Gene Polymorphisms, and Bladder Cancer Among Egyptian Agricultural Workers. Archives of Environmental & Occupational Health, 70 (1), 19–26. doi: https://doi.org/10.1080/19338244.2013.853646
Omidakhsh, N., Bunin, G. R., Ganguly, A., Ritz, B., Kennedy, N., von Ehrenstein, O. S. et. al. (2017). Parental occupational exposures and the risk of childhood sporadic retinoblastoma: a report from the Children’s Oncology Group. Occupational and Environmental Medicine, 75 (3), 205–211. doi: https://doi.org/10.1136/oemed-2017-104404
Depczynski, J. C. (2017). A population-based examination of cancer in New South Wales farm residents compared to rural non-farm and urban residents. University of Sydney. Available at: https://ses.library.usyd.edu.au/handle/2123/17114
Kim, K.-H., Kabir, E., Jahan, S. A. (2017). Exposure to pesticides and the associated human health effects. Science of The Total Environment, 575, 525–535. doi: https://doi.org/10.1016/j.scitotenv.2016.09.009
Benthiavalicarb-Isopropyl; pesticide tolerance (2006). Federal Register, 71 (170), 51998–52003. Available at: https://www.gpo.gov/fdsys/pkg/FR-2006-09-01/pdf/06-7313.pdf
De Albuquerque, N. C. P., Carrão, D. B., Habenschus, M. D., de Oliveira, A. R. M. (2018). Metabolism studies of chiral pesticides: A critical review. Journal of Pharmaceutical and Biomedical Analysis, 147, 89–109. doi: https://doi.org/10.1016/j.jpba.2017.08.011
Folpet; pesticide tolerance (2004). Federal Register, 69 (164), 52182–52192. Available at: https://www.gpo.gov/fdsys/pkg/FR-2004-08-25/pdf/04-19036.pdf
Ito, N., Shirai, T., Hasegawa, R.; Vainio, H., Magee, P. N., McGregor, D. B., McMichael, A. J. (Eds.) (1992). Medium term bioassays for carcinogens. Mechanism of carcinogenesis in riskidentification. The IARC Scientific Publications No. 93. Lyon: IARC, 353–388.
Sakthisekaran, D., Rajendran, P., Rengarajan, T., Nishigaki, I., Ekambaram, G. (2014). Potent chemopreventive effect of mangiferin on lung carcinogenesis in experimental Swiss albino mice. Journal of Cancer Research and Therapeutics, 10 (4), 1033. doi: https://doi.org/10.4103/0973-1482.137966
Wang, F., Zhu, Y., Zhou, L., Pan, L., Cui, Z., Fei, Q. et. al. (2015). Fluorescent In Situ Targeting Probes for Rapid Imaging of Ovarian-Cancer-Specific γ-Glutamyltranspeptidase. Angewandte Chemie International Edition, 54 (25), 7349–7353. doi: https://doi.org/10.1002/anie.201502899
McGlynn, K. A., Petrick, J. L., London, W. T. (2015). Global Epidemiology of Hepatocellular Carcinoma. Clinics in Liver Disease, 19 (2), 223–238. doi: https://doi.org/10.1016/j.cld.2015.01.001
Bagliy, Ye. A., Nedopitanska, N. M., Lisovska, V. S., Reshavska, O. V. (2011). Vplyv avermektyniv na iniciyovani nitrozodietylaminom gepatocyty shhuriv. Suchasni problemy toksykologiyi, 1, 22–26.
U.S. EPA (2017). Chemicals Evaluated for Carcinogenic Potential. Available: http://npic.orst.edu/chemicals_evaluated.pdf
Copyright (c) 2018 Sergii Omel’chuk, Alina Syrota
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.
Authors, who are published in this journal, agree to the following conditions:
1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the journal under the terms of a Creative Commons CC BY, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this journal.
2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form in which it has been published by the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.
